红树内生细菌RS261抗菌物质的发酵优化和性质分析  

Fermentation conditions and characteristics of the antibiotic active substances of the endophytic bacterial strain RS261

作　　者：柳凤[1] 卢仕严[1] 何红[1] 刘博凤[1] 欧雄常[1] 

机构地区：[1]广东海洋大学农学院,湛江524088

出　　处：《植物保护》2009年第5期70-75,共6页Plant Protection

基　　金：广东省自然科学基金项目(05011780);教育部科学技术研究重点项目(206106);广东省科技计划项目(2006b365001009)

摘　　要：RS261是一株来自红海榄（Rhizophora stylosa）叶片内对辣椒疫病具有良好防病效果的生防细菌。通过55％硫酸铵沉淀获得的抗菌蛋白液对辣椒炭疽病菌等多种植物病原菌具有强烈的抑制作用，该蛋白对热稳定、对蛋白酶K不敏感，但对胃蛋白酶和紫外线较敏感，在pH6．0～7．0时抗菌活性最强；优化后的培养基配方为：马铃薯200g／L、蛋白胨5g／L、蔗糖20g／L，适宜培养条件是：初始pH6．5、接种量5％、装液量为150mL／250mL、振荡培养72h时，产生的抗菌蛋白最多。The endophytic bacterial strain RS261 isolated from the leaves of Rhizophora stylosa was an efficient bio- logical control agent for controlling the capsicum phytophthora blight. Antagonistic crude protein extract was obtained through 55% ammonium sulphate precipitation. It was shown on PDA plates that the development of many pathogens such as Colletotrichum gloeosporioides was effectively inhibited by the antifungal protein. They had fairly good heat stability and were resistant to proteinase K, but susceptible to pepsin and UV. Their antibacterial activity was highest at pH6. 0 7. 0. Single-factor test results indicated that the optimal medial components for producing antifungal proteins were： 200 g/L potato, 5 g/L peptone and 20 g/L sucrose. The optimal culture conditions were： initial pH 6.5, 5% inoculation volume, 150 mL/250 mL transfer ratio and 72 h culture.

关 键 词：内生细菌 发酵 抗菌物质 

分 类 号：S436[农业科学—农业昆虫与害虫防治]