莪术油对肝癌细胞SMMC-7721基因表达的影响  

Effects of Turmeric rhizome Naphtha on Gene Expression of Hepatocarcinoma Cell SMMC-7721

作　　者：王顺启[1,2,3] 陈力[2] 倪虹[2] 穆亚丽[4] 刘玮[3] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南开大学生命科学学院,天津300071 [3]南昌大学生命科学与食品工程学院,江西南昌330031 [4]南昌大学计算机技术工程研究所,江西南昌330031

出　　处：《食品科学》2009年第19期240-243,共4页Food Science

基　　金：江西省教育厅科学技术研究项目(2007047);教育部"长江学者和创新团队发展计划"项目(IRT0540);中国博士后科学基金项目(20060390967)

摘　　要：目的:检测莪术油对肝癌细胞SMMC-7721生长,及肝癌相关基因表达的影响。方法:利用MTT比色法检测莪术油对肝癌细胞SMMC-7721增殖的影响;利用以肝癌cDNA抑制性消减文库构建的肝癌相关基因微阵列,检测莪术油作用肝癌细胞SMMC-772136h后基因的表达变化。结果:莪术油作用肝癌细胞SMMC-772136h后,细胞生长受到明显抑制,并观察到有23个基因表达下调,3个基因表达上调。结论:莪术油对肝癌肿瘤细胞的作用是通过影响多基因的表达改变实现的。Objective： To detect effects of Turmeric rhizome naphtha （TRN） on cell growth and gene expression of hepatocarcinoma cell SMMC-7721. Methods： Cell growth of hepatocarcinoma cell SMMC-7721 with TRN treatment was evaluated by MTT assays, and gene expression of hepatocarcinoma cell SMMC-7721 with TRN treatment for 36 hours was explored with microarray of hepatocarcinoma-associated genes, which resulted from suppression subtractive hybridization library. Results： After TRN treatment on hepatocarcinoma cell SMMC-7721 for 36 hours, cell growth was greatly inhibited and gene expression proftling analysis indicated that 23 genes were up-regulated and 3 genes were down-regulated. Conclusion： The effect of TRN treatment on hepatocarcinoma cell SMMC-7721 can be achieved through alteration of multiple genes expression, which provides important clues to understand the molecular mechanism of TRN effects on hepatocarcinoma ceils.

关 键 词：莪术油 肝癌细胞SMMC-7721 MTT比色法 微阵列 基因表达 

分 类 号：TS201.24[轻工技术与工程—食品科学]