白术多糖对局灶性脑缺血再灌注后诱导型一氧化氮合酶的影响  

Effect of Polysaccharide from Atractylodes macrocephala Koidz on Inducible Nitric Oxide Synthase after Focal Cerebral Ischemia Reperfusion in Rats

作　　者：王光伟[1] 丰昀[2] 刘永乐[3] 邱细敏[1] 

机构地区：[1]湖南师范大学医学院,湖南长沙410006 [2]长沙市中心医院,湖南长沙410004 [3]长沙理工大学生物与食品工程学院,湖南长沙410015

出　　处：《食品科学》2009年第19期273-275,共3页Food Science

基　　金：湖南省自然科学基金项目(08JJ3024);长沙市科技局科技计划资助项目(K069062-12)

摘　　要：目的:探讨局灶性脑缺血再灌注后应用白术多糖治疗对诱导型一氧化氮合酶(iNOS)的影响。方法:采用局灶性脑缺血再灌注模型,SpragueDawley大鼠随机分为对照组、再灌注组、蒸馏水组及白术多糖组,每组再根据不同的再灌注时间分为3个亚组。取缺血区组织,分别检测其水分含量及iNOS活性。结果:再灌注组、蒸馏水组及白术多糖组各时间点水分含量均较对照组明显增加(P<0.01),但白术多糖组各时间点水分含量显著低于再灌注组及蒸馏水组相应时间点(P<0.01)。再灌注组、蒸馏水组及白术多糖组各时间点iNOS活性均较对照组明显升高(P<0.01),但白术多糖组各时间点iNOS活性显著低于再灌注组及蒸馏水组相应时间点(P<0.01)。结论:白术多糖能下调缺血区iNOS的表达,减轻再灌注后脑水肿的程度。Objective： To study the effect of polysaccharide fromAtractylodes macrocephala Koidz on inducible nitric oxide synthase （iNOS） after focal cerebral ischemia reperfusion in rats. Methods： Eighty-four male Sprague Dawley rats were randomly divided into four groups： blank control, reperfusion model group, distilled water group and polysaccharide from Atractylodes macrocephala Koidz group. Meanwhile, three subgroups of rats were created in each group based on time intervals of reperfusion （24, 72 and 120 h） after 1 h of ischemia. Water content and iNOS activity in the ischemic brain tissues were determined. Results： Compared with the blank control group, water content revealed a significant increase in ischemic brain tissues from reperfusion model group, distilled water group and polysaccharide from Atractylodes macrocephala Koidz group at each time point （P 〈 0.01）. Similarly, iNOS activity also exhibited significantly higher in reperfusion model group, distilled water group and polysaccharide from Atractylodes macrocephala Koidz group than that of blank control group （P 〈 0.01）, however, the iNOS activity in polysaccharide from Atractylodes macrocephala Koidz group was significantly lower than that in reperfusion model group and distilled water group （P 〈 0.01）. Conclusion： Polysaccharide from Atractylodes macrocephala Koidz can dramatically inhibit iNOS expression and alleviate brain edema in ischemlc areas after focal cerebral ischemia reperfusion.

关 键 词：白术多糖 脑缺血 再灌注 一氧化氮合酶 

分 类 号：TS201.23[轻工技术与工程—食品科学]