Concerted stimuli regulating osteo-chondral differentiation from stem cells： phenotype acquisi- tion regulated by microRNAs  

作　　者：Jan O GORDELADZE Farida DJOUAD Jean-Marc BRONDELLO Daniele NOEL Isabelle DUROUX-RICHARD Florence APPARAILLY Christian JORGENSEN 

机构地区：[1]Department of Biochemistry, Institute for Basal Medical Sciences, Medical Faculty, University of Oslo, Norway [2]INSERM U844, Montpellier, France [3]Universite Montpellier 1, France [4]Unite Immuno-Rhumatologie Therapeutique, CHU Lapeyronie, Montpellier, France

出　　处：《Acta Pharmacologica Sinica》2009年第10期1369-1384,共16页中国药理学报（英文版）

摘　　要：Bone and cartilage are being generated de novo through concerted actions of a plethora of signals. These act on stem cells （SCs） recruited for lineage-specific differentiation, with cellular phenotypes representing various functions throughout their life span. The signals are rendered by hormones and growth factors （GFs） and mechanical forces ensuring proper modelling and remodelling of bone and cartilage, due to indigenous and programmed metabolism in SCs, osteoblasts, chondrocytes, as well as osteoclasts and other cell types （eg T helper cells）. This review focuses on the concerted action of such signals, as well as the regulatory and/or stabilizing control circuits rendered by a class of small RNAs, designated microRNAs. The impact on cell functions evoked by transcription factors （TFs） via various signalling molecules, also encompassing mechanical stimulation, will be discussed featuring microRNAs as important members of an integrative system. The present approach to cell differentiation in vitro may vastly influence cell engineering for in vivo tissue repair.Bone and cartilage are being generated de novo through concerted actions of a plethora of signals. These act on stem cells （SCs） recruited for lineage-specific differentiation, with cellular phenotypes representing various functions throughout their life span. The signals are rendered by hormones and growth factors （GFs） and mechanical forces ensuring proper modelling and remodelling of bone and cartilage, due to indigenous and programmed metabolism in SCs, osteoblasts, chondrocytes, as well as osteoclasts and other cell types （eg T helper cells）. This review focuses on the concerted action of such signals, as well as the regulatory and/or stabilizing control circuits rendered by a class of small RNAs, designated microRNAs. The impact on cell functions evoked by transcription factors （TFs） via various signalling molecules, also encompassing mechanical stimulation, will be discussed featuring microRNAs as important members of an integrative system. The present approach to cell differentiation in vitro may vastly influence cell engineering for in vivo tissue repair.

关 键 词：growth factors transcription factors mechano-stimulation MICRORNAS stem cells OSTEOBLASTS CHONDROCYTES tissue engineering 

分 类 号：Q522[生物学—生物化学] Q813.11