呋喃唑酮代谢物荧光纳米颗粒免疫层析法的建立  

Determination of AOZ with Fluorescent Nanoparticles Based Lateral Flow Strip Assay

作　　者：朱海[1] 范放[1] 吕敬章[1] 赵芳[1] 万志刚[1] 马淑棉[1] 司徒翠华[1] 

机构地区：[1]深圳出入境检验检疫局,深圳518067

出　　处：《畜牧与饲料科学》2009年第6期32-34,共3页Animal Husbandry and Feed Science

基　　金：深圳出入境检验检疫局科技计划项目(SZ2007042)

摘　　要：研究将荧光纳米颗粒与呋喃唑酮代谢物单克隆抗体共价耦联在一起,以竞争法作为反应模式来制备呋喃唑酮代谢物免疫层析试纸条。所制备的免疫层析试纸条仅与呋喃唑酮代谢物AOZ有交叉反应,而与非目标检测物之间无交叉反应,其灵敏性达1.0ng/mL,可满足相关检测标准要求。并且通过肉眼观察检测线和质控线之间的亮度,还可对AOZ进行半定量检测。该试纸条可用于食品中呋喃唑酮代谢物的检测。In this study, an lateral flow strip for the determination of Furahadone Metabolin（FM-AOZ）was made. A monoclonal antibody against （FM-AOZ） was covalcntly conjugated with fluorescent nanoparticle, and was employed in the development of lateral flow strip assay. Crossing-reaction could be emerged between the prepared strip and FM-AOZ, but it didn＇t react to others. It could satisfy the need of detection with1.0 ng/mL of its sensitivity, meanwhile it also could be used semi-quantitative test of FM-AOZ through observing the brightness between detection line and quality control line with eye. The conclusion was made that FM-AOZ from foods can be detected by it.

关 键 词：单克隆抗体 纳米颗粒 免疫层析试纸条 呋喃唑酮代谢物 

分 类 号：TS207.5[轻工技术与工程—食品科学]