肝失疏泄—脂质代谢异常动物模型的初步研究  

Preliminary Study on the Animal Model of Lipid Metabolism Abnormality Basing on Not Liver Freely Distributing

作　　者：闫智勇[1] 张天娥[2] 肖婷[1] 左长英[1] 秦晋之[1] 潘玲珍[1] 张占平[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031 [2]成都中医药大学,四川成都610075

出　　处：《中华中医药学刊》2009年第10期2229-2231,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:研究建立肝失疏泄—脂质代谢异常动物模型。方法:根据祖国医学肝失疏泄,致水谷精微失于正常输布、转化的病机特点,采用慢性应激辅助高脂饮食的复合方法,以大鼠的行为学和血脂代谢水平为观测指标,研究建立肝失疏泄—脂质代谢异常动物模型。结果:行为学观察表明,在肝失疏泄对照组,大鼠出现比较典型的肝失疏泄表现,而在复合造模组(肝失疏泄—脂质代谢异常模型组),大鼠的行为学表现则兼有肝失疏泄和高脂饮食所造成的症状特点。血脂检测结果表明,正常对照组和肝失疏泄组的血清TG、TC、HDL-C和LDL-C含量都没有显著性差异,而高脂饮食组和复合造模组的TC、HDL-C和LDL-C含量则显著高于正常对照组(P<0.05或P<0.01或P<0.001),其中复合造模组的TC、LDL-C含量和高脂饮食组相比有明显差异(P<0.05,P<0.01)。结论:应用慢性应激加高脂饮食辅助的方法,初步建立了肝失疏泄—脂质代谢异常动物模型。Objective： To establish the animal model of lipid metabolism abnormality basing on not liver freely distributing. Methods ：The composite method of chronic stress and high fat diet were used to study the animal model basing on the pathogenesis features in the Traditional Chinese Medicine. The theory thought the spreading and transformation of food essence could be effected by not liver freely distributing. The ethology and serum TG, TC, HDL - C and LDL - C were observed. Results ：The observation of ethology indicated that the rats had the typical appearance of not liver freely distributing in the not liver freely distributing group. In the composite method model group, the rats simultaneously appeared the characteristics of not liver freely distributing and high fat diet. The contents of serum TG,TC ,HDL - C and LDL - C were not obvious different between normal control group and not liver freely distributing group. But compared with normal control group, the contents of TC, HDL - C and LDL - C were obviously improved in high fat diet group and composite method model group（P 〈 0.05 or P 〈 0.01or P 〈 0. 001 ）. Furthermore the contents of TC and LDL - C in composite method model group were obviously superior to that in high fat diet group（ P 〈 0.05 ,P 〈 0.01 ）. Conclusion ：The animal model of lipid metabolism abnormality basing on not liver freely distributing has been initially established by the composite method of chronic stress and high fat diet.

关 键 词：肝失疏泄 脂质代谢 动物模型 

分 类 号：R-33[医药卫生]