一种快速简单显示神经元树突棘的Golgi-Cox法(英文)  

A rapid and simple Golgi-Cox method to label dendritic spines

作　　者：张富兴[1] 董加强[2] 张卓超[2] 刘立鹏[2] 李云庆[1] 

机构地区：[1]第四军医大学人体解剖与组织胚胎学教研室,西安710032 [2]第四军医大学口腔医学系十队,西安710032

出　　处：《神经解剖学杂志》2009年第5期497-500,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30470562)资助项目

摘　　要：现存很多高尔基染色法有成功率低、产生沉淀或操作程序复杂等缺点。本文报道了我们发展的显示大脑皮层神经元树突棘的另一种Gogli-Cox染色方法。其方法是将动物灌注固定后取脑并将全脑浸于Golgi-Cox液中2周,然后浸于30%蔗糖中;反应采用100微米厚的震动切片,经过蒸馏水洗涤、氨化、酸性坚膜定影液反应、蒸馏水再洗涤,然后切片经上升梯度酒精脱水、透明,裱片后观察。结果显示,神经元的形状和树突棘标记十分清楚;相比之下,我们采用的另一种应用K2S的Golgi-Cox法则标记质量很差。本研究结果提示我们使用酸性坚膜定影液的变通Golgi-Cox染色法是一种简单而有效的标记神经元树突棘的方法。The Golgi methods currently available have disadvantages such as low successful rate, production of precipitate or involving complex procedures. This paper reports our study by using a modified Golgi-Cox method to demonstrate dendritic spines of cerebral cortex neurons. Briefly, animal was fixed by perfusion and the whole brain was removed. After immersion in Golgi-Cox solution for two weeks, the brain was transferred to sucrose. Slices of 100μm in thickness were cut on vibratome and were sequentially processed through distilled water, ammonium, acidic hardening fixing solution, distilled water; then slices were dehydrated and cleared through ascending concentration of alcohol and xylene before mounting and coverslipping for observation. The results showed that neuronal profiles and dendritic spines were perfectly labeled and the sections had no background staining, while the other Golgi-Cox using K2S method as we used in the study showed labeling of poor quality. It is suggested that our modified Gogli-Cox method using acidic hardening fixing solution is a simpler and efficient way to label dendritic spines.

关 键 词：树突棘 高尔基染色 大脑皮质 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]