抗黄曲霉毒素B_1单抗独特型抗体的制备  

Preparation of Polyclonal Anti-Idiotypic Antibody Against Anti-Aflatoxin B_1 Monoclonal Antibody

作　　者：江涛[1] 柳桢[1] 李楠[1] 李燕俊[1] 高秀芬[1] 计融[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国食品卫生杂志》2009年第5期385-389,共5页Chinese Journal of Food Hygiene

基　　金："十五"国家科技重大专项(2001BA804A46)

摘　　要：目的研制黄曲霉毒素B1标准品的替代品并应用于酶联免疫吸附试验(ELISA)。方法在研制了抗黄曲霉毒素B1单抗的基础上,将抗体用无花果蛋白酶进行酶切,制备出抗黄曲霉毒素B1单抗F(ab’)2片段,并以F(ab’)2片段作为免疫原免疫日本大耳白兔,制备出抗黄曲霉毒素B1单抗独特型抗体,并对该独特型抗体进行鉴定。结果该独特型抗体是Ab2β型,与黄曲霉毒素B1存在内影像关系。结论该抗体可以替代黄曲霉毒素B1标准品应用于ELISA检测。Objective To prepare a substitute for aflatoxin B1 standard and to apply it for enzyme-linked immunosorbent assay （ELISA）. Method The polyclonal anti-idiotype antibody against anti-aflatoxin B1 monoclonal antibody was prepared by immunizing Japanese flap-eared rabbits with F（ab ）2 fragment of anti-aflatoxin B1 monoclonal antibody.Results The polyclonal anti-idiotype antibody was identified as a Ab2β subclass and as an ＂inner image＂ with aflatoxin B1. The polyclonal anti-idiotype antibody can be used as a substitute for aflatoxin B1 standard in ELISA.

关 键 词：黄曲霉毒素B1 抗体 抗独特型 酶联免疫吸附测定 

分 类 号：R392[医药卫生—免疫学] R155.5[医药卫生—基础医学]