发酵产菊糖果糖转移酶的培养基设计及菌体生长动力学研究  

Optimization of Inulin Fructotransferase-producing Medium and Determination of Cell Growth Kinetic Parameter for Arthrobacter ureafaciens SK-8.001

作　　者：卢峥嵘[1] 赵萌[1] 沐万孟[1] 张涛[1] 江波[1] 

机构地区：[1]江南大学食品学院食品科学与技术国家重点实验室,江苏无锡214122

出　　处：《食品与发酵工业》2009年第9期19-24,共6页Food and Fermentation Industries

基　　金："十一五"国家863资助项目(2006AA10Z334)

摘　　要：对1株产菊糖果糖转移酶(inulin fructotransferase)的金黄色节杆菌(Arthrobacter ureafaciens)SK-8.001的产酶培养基进行了优化,同时对其在分批发酵过程中培养基组分变化和菌体生长动力学进行了研究。所得到的改进型产酶培养基配方为25 g/L菊糖,3 g/L NaNO3,1 g/L酵母膏,0.1 g/L MgSO4,0.4 g/L KH2PO4,0.01 g/LFeSO4.7H2O。在此培养基中的最高酶活达到19.85 U/mL,较初始培养基提高了2.04倍。对在此培养基中Sk-8.001的发酵过程研究发现发酵12 h和66 h是较为理想的诱导物添加时刻。得到SK-8.001在优化培养基中分批培养的菌体生长动力学模型为:X(t)=9.356 05/[1+exp(4.4222-0.1102t)]。In this paper, the optimal inulin fructotransferase-producing medium and fermentation kinetic parameters of Arthrobacter ureafaciens SK- 8. 001 were studied. The recipes of optimal media included 2. 5% inulin,0.3% NaNO3,0. 1%yeast extract,0. 01% MgSO4,0. 04% KH2PO4,0.001% FeSO4·7H2O. The maximum enzyme activity reached 19. 85 U/mL, which was 2. 04 fold higher than in initial media. The study on the fermentation process in the optimal media suggested the right time to add inducers into the media was 12 h or 66th hours during the fermentation. The cell growth kinetic model for SK-8.001 in batch fermentation was X （t） =9. 356 05/ [ 1 ＋ exp（4.4222-0.11-2） ].

关 键 词：DFAⅢ 菊糖果糖转移酶 金黄色节杆菌 菌体生长动力学 

分 类 号：TS241[轻工技术与工程—制糖工程]