木聚糖酶酶活性测定方法及酶活性单位定义  

Assay Methods and Unit Definitions of Xylanase Activity

作　　者：王晓丹[1] 郭丽琼[1,2] 赵力超[1] 林俊芳[1,2] 

机构地区：[1]华南农业大学食品学院生物工程系,广东广州510640 [2]华南农业大学生物质能研究所,广东广州510640

出　　处：《食品与发酵工业》2009年第9期128-131,共4页Food and Fermentation Industries

基　　金：国家高技术研究发展计划(863计划)课题(2006AA10Z301);广东省科技攻关项目(2008B0000005)

摘　　要：为统一木聚糖酶酶活性的单位定义及测定方法进行了实验。通过对现有酶活性的单位定义及测定方法的比较分析,得出木聚糖酶的酶活性单位定义为:在特定条件下,每分钟水解木聚糖形成1μmol木糖(还原糖)所需酶量为1个酶活力单位(U),并且采用还原糖法中的DNS法作为测定木聚糖酶酶活性的方法,该法具有合理性和科学性,建议以此作为木聚糖酶酶活性测定及酶活性单位定义的统一方法。The definition and determination of xylanase activity was unified in this article. Through summarizing and comparing the present knowledge on different procedure for the determination of xylanase activity, one unit of xylanase activity was defined as the quantity of enzyme required to liberate 1μmol of reducing sugar （ as xylose） per minute at the measure conditions . This DNS method of expressing xylanase activity is scientifically sound and we suggest that this method be utilized as the universal and standard method of determining xylanase activity.

关 键 词：木聚糖酶 酶活性测定 酶活性单位定义 

分 类 号：TS201.25[轻工技术与工程—食品科学]