野生型p53对小鼠纤维肉瘤细胞线粒体功能的影响  

The influence of Wtp53 gene on cytochondriome function of fibrosarcoma suppression in mouse

作　　者：高峰[1] 潘海乐[1] 曲波[1] 田军[1] 陶天遵[1] 

机构地区：[1]哈尔滨医科大学附属第二医院急诊创伤外科,尔滨150086

出　　处：《实用肿瘤学杂志》2009年第5期414-416,共3页Practical Oncology Journal

摘　　要：目的观察野生型p53（Wtp53）基因对纤维肉瘤细胞线粒体结构和功能的影响，探讨Wfp53的抑癌机制。方法将5周龄昆明鼠随机分为单纯肿瘤组（对照组）和wt053基因治疗组（治疗组），建立纤维肉瘤动物模型，5d后治疗组动物于荷瘤局部注射表达WIp53基因的重组腺病毒液0．1mL，对照组同一部位注射等剂量生理盐水。两组动物于2次注射后10d、20d、30d分批处死，量取肿瘤直径大小，Estabrook法分离提取线粒体，Clark氧电极法进行线粒体呼吸功能的测定，Reer法测定游离Ca^2＋浓度。结果对照组肿瘤的凋亡指数明显低于治疗组。对照组中R3显著高于治疗组；10天时两组R4接近，但20天和30天时治疗组R4时相大大延长，与对照组比较差异非常显著；对照组RCR随着时间的延长无明显变化，治疗组中，RCR随时间延长而下降明显低于对照组；游离Ca^2＋浓度在10天时与对照组接近，而后大幅度上升。结论wtp53明显降低线粒体呼吸功能，增加线粒体的通透性，从而在促进细胞凋亡方面发挥对纤维肉瘤的治疗作用。Objective To investigate the influence of Wtp53 gene on cytochondriome function of fibrosarcoma in mouse. Methods Ninety Kunming mice （5weeks）were randomly divided into two groups, control group and Wtp53 - therapy group. Kunming mice were subcutaneously injected with S180 fibrosarcoma suspension liguor（0.1 mL）at femur of posterior limb. Five days after first injection,the mice were injected with sterile saline water（0.1 mL）and Wtp53 adenovirus liguor respectively at the first - injection area. Two groups of mice were separately killed at 10 d,20 d and 30 d after the second injection. Drawing out of the cytochondriome with Eseabroob method, respiration of cytochondriome was measured using the method of Clark,Ca^2＋ with Reer method. Results Apoptosis index in control group was lower than that in therapy group. R3 in control group was significantly higher than that in therapy group ; R4 of the two groups were closely in 10 days while extended in 20 or 30 days in therapy group,and there existed significantly difference;RCR in control group had not been found obvious change; while in therapy group RCR decreased sharply than that in therapy group,free concentration of Ca^2＋ was similar during the 10 days and then increased clearly. Conclusion Wtp53 can obviously reduce the respiration of cytochondriome,increased the penetration of cytochondriome and suppress tumor through reducing proliferation and inducing apoptosis of tumor cells.

关 键 词：WTP53 纤维肉瘤 线粒体 

分 类 号：R738.1[医药卫生—肿瘤]