假丝酵母菌GXU08产脂肪酶发酵条件的优化  

The optimization of fermentation conditions of Candida sp.GXU08 lipase

作　　者：沈芳[1] 刘雄民[1] 刘力恒[2] 黄荣韶[3] 李志刚[3] 

机构地区：[1]广西大学化学化工学院,南宁530004 [2]玉林师范学院化学与生物系,广西玉林537000 [3]广西大学农学院,南宁530004

出　　处：《广西植物》2009年第5期689-693,共5页Guihaia

基　　金：广西大学科研基金(DD040045)~~

摘　　要：以假丝酵母菌GXU08产脂肪酶催化合成麝香类香料—环十五内酯目前已备受关注,在一定条件下,环十五内酯的转化率与脂肪酶的水解酶活有直接关系,酶活越高其催化合成环十五内酯的能力越强。通过单因素试验和正交试验,对假丝酵母菌GXU08产脂肪酶的发酵条件进行优化。结果表明:最佳发酵培养基配方为蔗糖0.5%,淀粉0.5%,蛋白胨1.5%,K_2HPO_40.05%,MgSO_40.15%,(NH_4)_2SO_41%,茶油1.5%,菜籽油1.5%,pH=8,此培养基在28℃,180 r/min的条件下发酵培养48h,脂肪酶水解活力达到27.53 U/mL,是初始发酵培养基条件下所得脂肪酶酶活的3.74倍;其环十五内酯的转化率为16.6%,是优化前的4倍。The synthesizing of cyclopentadecanolide（a kind of macrocycle musk）by Candida sp. GXU08 lipase has been concerned. Under certain conditions,the converse of cyclopentadecanolide was directly connected with lipase activity. The higher the lipase activity was, the stronger the conversions were. The fermentation conditions of Candida sp. GXU08 lipase were optimized through the single factor and the orthogonal experiments. The results showed that： The compositions of optimal fermentation medium were sucrose 0. 5%, starch 0. 5%, peptone 1.5%, K2HPO4 0.05% ,MgSO4 0.15%, （NH4）2SO4 1% ,camellia oil 1.5%, rapeseed oil 1.5% ,pH=8.0 Under the fermentation condition of 28 ℃, 180 r/min,48 h, the lipase activity would reach 27.53 U/mL, which was 3.74 times of the original culture medium. The conversion of cyclopentadecanolide was 16.6%,which was 4 times of unoptimized.

关 键 词：假丝酵母菌 脂肪酶 发酵条件 正交实验 环十五内酯 

分 类 号：Q789[生物学—分子生物学]