辐照对糖多孢红霉菌M的诱变效应  

EFFECTS OF ^(60)Co γ-rays IRRADIATION ON Saccharopolyspora erythraea M

作　　者：庄韬[1,2] 刘超[2] 武杉杉[1,2] 黄训端[1] 张部昌[1] 曹磊[2] 张福生[2] 汪晓鸣[2] 

机构地区：[1]安徽大学生命科学学院,安徽合肥230039 [2]安徽省农业科学院原子能应用研究所,安徽合肥230031

出　　处：《核农学报》2009年第5期773-777,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(30670068);安徽省科技厅重点科研项目(08020303057);安徽省教育厅自然科学基金(KJ2007A017)

摘　　要：采用不同剂量的60Co γ射线辐照糖多孢红霉菌M孢子,并采用不同的菌种保护剂,以研究60Co γ射线对糖多孢红霉菌M的诱变效应。结果表明,使用20%甘油吐温保护效果较好,可作为诱变保护剂;当辐照剂量为400Gy时,致死率为90%左右可作为适宜诱变剂量。诱变处理后利用链霉素抗性突变选育得到1203株链霉素抗性突变株。其中5株形态发生较明显的变化,形态变异率为4.16‰;2株突变株的发酵液具有抑菌活性,正突变率为1.67‰。Spores of Saccharopolyspora erythraea M with different protective agents were treated with different doses of ^60 Co γ-rays irradiation, and their morphological variations were observed and mutants were selected. The results indicated that 20% glycerol （contain 0.1% tween-20） showed a better protective effect to irradiation, and lethal ratio reached 90% when irradiation dose at 400 Gy, therefore 400 Gy was selected as the appropriate dose. 1203 strains were obtained by streptomycin resistance screening, among them, 5 strains changed in their phenotype and 2 strain fermentation products had antibacterial activity. The morphological variation rate was 4‰ and positive mutation rate was 1.67‰.

关 键 词：60Coγ射线 糖多孢红霉菌M 诱变效应 链霉素抗性 

分 类 号：Q933[生物学—微生物学]