重组植酸酶高表达与新型智能称量式补料装置的工业应用  

High-level expression of recombinant phytase in Pichia pastoris and industrial application of novel intelligent fed-batch weighing device

作　　者：刘健[1] 张伟平[1] 张恂[1] 张明[1] 郭美锦[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心,上海200237

出　　处：《中国抗生素杂志》2009年第10期602-605,共4页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(No.30560006);上海市生物过程工程平台研究项目(07DZ22914)

摘　　要：针对重组毕赤酵母（Pichia pastoris GS115-phy）表达的基因工程产品的高吨位发酵罐的工业生产，采用称重传感器与计算机组合方式设计并制造了一新型智能称量式补料系统，具有高精度和重复性。智能称量式补料装置与电子秤对比试验误差均在-0．001—0．009kg之间，相对误差小于0．5％。将智能称量式补料装置应用于重组植酸酶发酵，很好地控制了甲醇补料速率，在25m3发酵罐中211h植酸酶表达量达到23，077U／ml的高水平。This paper aimed at high-level expression of recombinant product by yeast Pichia pastoris GS115- phy in industrial-scale fermenter. A novel intelligent fed-batch weighing device was designed and manufactured based on an integration of weighing sensor and personal computer, which is of high precision and reproducibility. For the intelligent fed-batch weighing device, absolute experimental errors ranging from - 0. 001 to - 0.009 kg and relative errors of less than 0. 5% was achieved, respectively, as compared with electronic scale device. Meanwhile, the intelligent fed-batch weighing device was applied to control methanol feeding rate in genetically engineered phytase expression by yeast Pichia pastoris GS115-phy. Recombinant phytase yield of 23077U/ml was reached in 25m3 manufacturing plant at 211h when the intelligent fed-batch weighing device was used.

关 键 词：智能称量式补料装置 重组植酸酶 工业应用 

分 类 号：TQ925[轻工技术与工程—发酵工程]