骨髓涂片荧光原位杂交法在检测多发性骨髓瘤细胞8号染色体遗传学异常的应用  

Fluorescence In Situ Hybridization on Bone Marrow Smear in the Detection of Cytogenetic Aberrations of Multiple Myeloma

作　　者：王晓炜[1,2] 李建勇[1] 陈丽娟[1] 钱思轩[1] 洪鸣[1] 乔纯[1] 张建富[1] 徐卫[1] 陆化[1] 许家仁[1] 

机构地区：[1]南京医科大学第一附属医院、江苏省人民医院血液科,江苏南京210029 [2]南京医科大学附属淮安第一医院肿瘤内科,江苏淮安223300

出　　处：《中国实验血液学杂志》2009年第5期1230-1233,共4页Journal of Experimental Hematology

基　　金：江苏省社会发展基金资助项目(编号BS2006071);江苏省高校自然科学基金资助项目(编号07KJB320074);卫生部科研基金(编号WKJ2007-3-001);江苏省自然科学基金资助项目(编号BK2008465)

摘　　要：本研究旨在建立骨髓涂片间期荧光原位杂交(I-FISH)的实验方法,为检测多发性骨髓瘤(MM)分子细胞遗传学提供新方法。以骨髓涂片为载体,通过一系列的处理,固定及消化后,用8号染色体着丝粒探针行I-FISH分子细胞遗传学的检测,并比较该方法与常规I-FISH结果的差异。结果表明:两种方法分析非恶性血液病标本的各个信号比例无统计学差异(p>0.05)。骨髓涂片I-FISH研究中,19例MM患者中8例(42.1%)有8号染色体异常,其中8号染色体单体(-8)5例(26.3%),8号染色体三倍体(+8)3例(15.8%)。结论:骨髓涂片I-FISH具有操作简便、经济、准确的特点,可用于MM分子遗传学异常的研究。This study was aimed to establish the technique of interphase fluorescence in situ hybridization （I-FISH） used on smear of bone marrow directly, and to develop a new method for detection of the molecular cytogenetics in multiple myeloma （MM）. After a series of treatment,fixation and digestion of the bone marrow smear as the carrier, the chromosome 8 centromere probe were used in I-FISH for molecular cytogenetics detection. At the same time, differences were compared in the results between the new method and the conventional I-FISH. The results showed that there was no statistically significant difference of proportion of various signals in non-hematologic malignancies when detecteding with the two methods （p 〉 0. 05）. In bone marrow smear I-FISH, 8 out of 19 cases （42. 1% ） had abnormality of chromosome 8, including 5 cases with - 8 （ 26.3% ） and 3 cases with ＋ 8 （ 15.8% ）. It is concluded that the I-FISH on smear of bone marrow is characterized by convenience, economy and accuracy. Therefore, it can be used for research of molecular cytogenetics in MM.

关 键 词：骨髓涂片 荧光原位杂交 多发性骨髓瘤 8号染色体 

分 类 号：R733.3[医药卫生—肿瘤] R446[医药卫生—临床医学]