Bioinformatic analysis of microRNA biogenesis and function related proteins in eleven animal genomes  

作　　者：Xiuying Liu GuanZheng Luo Xiujuan Bai Xiu-Jie Wang 

机构地区：[1]College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China [2]State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [3]Graduate University of Chinese Academy of Sciences, Beijing 100101, China d College of Life Science, Northeast Agricultural University, Harbin 150030, China

出　　处：《Journal of Genetics and Genomics》2009年第10期591-601,共11页遗传学报（英文版）

基　　金：supported by grants from the Ministry of Science and Technology of China (No. 2007CB946901);the Natural Science Foundation of China (No. 30725014 and 90612019);the Chinese Academy of Sciences (No. KSCX2-YW-R-134)

摘　　要：MicroRNAs are -22 nt long small non-coding RNAs that play important regulatory roles in eukaryotes. The biogenesis and functional processes of microRNAs require the participation of many proteins, of which, the well studied ones are Dicer, Drosha, Argonaute and Exportin 5. To systematically study these four protein families, we screened 11 animal genomes to search for genes encoding above mentioned proteins, and identified some new members for each family. Domain analysis results revealed that most proteins within the same family share identical or similar domains. Alternative spliced transcript variants were found for some proteins. We also examined the expression patterns of these proteins in different human tissues and identified other proteins that could potentially interact with these proteins. These findings provided systematic information on the four key proteins involved in microRNA biogenesis and functional pathways in animals, and will shed light on further functional studies of these proteins.MicroRNAs are -22 nt long small non-coding RNAs that play important regulatory roles in eukaryotes. The biogenesis and functional processes of microRNAs require the participation of many proteins, of which, the well studied ones are Dicer, Drosha, Argonaute and Exportin 5. To systematically study these four protein families, we screened 11 animal genomes to search for genes encoding above mentioned proteins, and identified some new members for each family. Domain analysis results revealed that most proteins within the same family share identical or similar domains. Alternative spliced transcript variants were found for some proteins. We also examined the expression patterns of these proteins in different human tissues and identified other proteins that could potentially interact with these proteins. These findings provided systematic information on the four key proteins involved in microRNA biogenesis and functional pathways in animals, and will shed light on further functional studies of these proteins.

关 键 词：microRNA DROSHA DICER ARGONAUTE Exportin 5 

分 类 号：Q522[生物学—生物化学] S829.12[农业科学—畜牧学]