微波诱变L(+)-乳酸高产菌株的选育  

Screening of High-yeild L(+)-Lactic Acid Strains by Microwave Irradiation Mutagen

作　　者：李市场[1] 朱朝阳[1] 王敏[1] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471003

出　　处：《激光生物学报》2009年第5期647-651,共5页Acta Laser Biology Sinica

基　　金：河南科技大学科学研究基金项目(2006QN007)

摘　　要：为了获得L-乳酸高产菌株,对干酪乳杆菌(Lactobacillus casei)CICC6028进行微波诱变。通过考察微波辐照功率和时间等因素对诱变结果的影响,得出最佳诱变方法是:间歇诱变方法,微波输出功率480 W,辐照时间40 s。最后得到一株遗传稳定的L(+)-乳酸高产菌株MW-46,产酸量最高为98.12 g/L,与出发菌相比,提高了54.14%。In order to obtain the strain with high-yield L （ ＋）-lactic acid, the original strain, Lactobacillus casei CLCC6028, was mutated by microwave. The irradiation power and time of microwave were studied, and the optimum mutagenie method was, irradiation power 480 W and mutation time 40 s. Finally, the high-yield lactic aid strain MW-46 was obtained, and the highest yield was 98.12 g/L, increased by 54.14 % compared with the original strain.

关 键 词：干酪乳杆菌 微波 L(+)-乳酸 选育 

分 类 号：Q631[生物学—生物物理学] Q933