高效利用木糖产油的氮离子注入Mortierella alpina诱变选育研究  

Efficient Use of Xylose for Microbial Lipids Production with Mortierella alpina by N^+ Ion Implantation

作　　者：张腊梅[1] 袁成凌[1] 李娟[1] 王鹏[1] 王丽[1] 郑之明[1] 

机构地区：[1]中国科学院离子束生物工程学重点实验室,安徽合肥230031

出　　处：《激光生物学报》2009年第5期673-681,共9页Acta Laser Biology Sinica

基　　金：国家"十一五"863计划重点项目(2006AA020101);中国科学院合肥物质科学研究院院长基金项目(085FZ10133)

摘　　要：木糖的有效利用是木质纤维素全利用的基础。为了获得高效利用木糖产油的高山被孢霉菌株,通过多轮氮离子束诱变,筛选出一株有效利用木糖的产油高山被孢霉I502-8(Mortierella alpina I502-8,MAI502-8),并研究了以葡萄糖/木糖(W/W,5/3)组成的混合糖为碳源,突变株生长和油脂积累的特性。采用单因子和正交实验对培养基组成和发酵条件进行了优化,结果表明,诱变菌在温度28℃,pH 8.0,接种量8%,装液量30%,蛋白胨0.76%和豆粕1%,分批补糖的最优发酵条件下发酵9d,生物量和菌体油脂积累量分别达29.8g/L和11.7g/L,较出发菌提高了2.59倍和2.05倍,同时原料糖利用率达到99.4%。Efficient use of xylose is the basis for using lignocellulose completely. To obtain an industrial strain with a higher microbial lipids yield, the strain Mortierella alpina was mutated through many times of nitrogen ion beam implantations and the mutant strain Mortierella alpina 1502-8 was obtained. The properties of growth and lipids accumulation from glucose and xylose （W/W, 5/3） were also studied. The medium composition and some fermentation parameters were studied by cross test and single factor experiments. The optimal conditions are as follows： tryptone 0.76 %, bean powder 1.0 % ,initial pH 8.0, temperature 28 ℃ , inoculation quantity 8 % and loading volume 30 %. Under optimal conditions, the maximum yield of biomass and microbial lipids reached 29.8 g/L and 11.7 g/L, respectively, which was 2.59 times and 2.05 times of those cultured in the original medium, after 9 days fed-batch co-fermentation in shakeflask. And 99.4 % of the mixed sugar used up during co-fermentation.

关 键 词：氮离子注入 高山被孢霉 混合糖 微生物油脂 补料分批发酵 

分 类 号：Q319[生物学—遗传学]