CyclinB1、CDK1在食管鳞癌组织中的表达及临床意义  

Clinical significance of cyclin B1 and cyclin-dependent kinase 1 expression in human esophageal squamous cell carcinoma

作　　者：赵春玲[1] 陈丽梅[1] 高志芹[1] 杜长青[1] 潘智芳[1] 于文静[1] 

机构地区：[1]潍坊医学院细胞生物学教研室,山东省潍坊市261053

出　　处：《世界华人消化杂志》2009年第23期2374-2378,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨食管鳞癌组织中细胞周期蛋白CyclinB1和细胞周期蛋白依赖性激酶CDK1表达及其临床病理学意义.方法:应用免疫组织化学SP法对52例食管鳞癌(组织学Ⅰ级8例,Ⅱ级20例,Ⅲ级24例;有淋巴结转移20例,无淋巴结转移32例;原位癌16例,侵袭性癌36例包括浸润至黏膜下层、肌层、全层)及其配对的癌旁正常组织进行CyclinB1、CDK1的检测,分析其阳性表达与食管鳞癌患者临床病理因素的关系.结果:食管鳞癌组织中CyclinB1、CDK1的阳性表达高于癌旁正常食管黏膜组织,2组差异都有统计学意义(71.2%vs2.0%,65.4%vs3.9%,均P<0.05).食管鳞癌组织中CyclinB1、CDK1的表达都与性别、年龄无关;与组织学分级、浸润深度及淋巴结转移有关(均P<0.05).CyclinB1阳性表达强度与CDK1的阳性表达强度之间呈正相关(r=0.697,P<0.05).结论:CyclinB1、CDK1的高表达会促进食管鳞癌的发生与发展.而且食管鳞癌中CyclinB1与CDK1的表达密切相关,可作为食管鳞癌生物学行为预测的参考指标.AIM： To investigate the expression of cyclin B1 and cyclin-dependent kinase 1 （CDK1） proteins in esophageal squamous cell carcinoma （ESCC） and analyze its clinicopathological signif icance. METHODS： A total of 52 ESCC specimens were used in the study. Matched normal esophageal mucosal tissues were used as controls. Of all ESCC specimens, 8 had grade Ⅰdisease, 20 had grade Ⅱdisease, 24 had grade Ⅲ disease; 20 had lymph node metastasis, 32 had no lymph node metastasis; 16 had carcinoma in situ, 36 had invasive carcinoma. The expression of cyclin B1 and CDK1 was detected by streptavidinperoxidase （SP） immunohistochemical method. The correlation of cyclin B1 and CDK1 expression with the clinical features of ESCC was then analyzed.RESULTS： The expression levels of cyclin B1 and CDK1 were significantly higher in ESCC tissues than in adjacent normal esophageal mucosal tissues （71.2% vs 2.0% and 65.4% vs 3.9%, respectively; both P〈0.05）. The overexpression of cyclin B1 and CDK1 in ESCC tissues had no correlation with patient gender and age, but had significant correlations with histological grade, depth of invasion and lymph node metastasis （all P〈0.05）. The overexpression of cyclin B1 in ESCC tissues was positively correlated with that of CDK1 （r = 0.697, P〈0.05）. CONCLUSION： Overexpression of cyclin B1 and CDK1 proteins can promote the development and progression of ESCC. There is a positive correlation between the expression of cyclin B1 and CDK1 in ESCC. Detection of cyclin B1 and CDK1 expression is useful for evaluation of the biological behavior of ESCC.

关 键 词：食管鳞癌 CYCLINB1 细胞周期蛋白依赖性激酶 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]