PTEN对鳞癌细胞的抑制作用及其在口腔白斑和鳞癌中的表达  

Expressions of PTEN in oral leukoplakia and oral squamous cell carcinoma and its inhibitory effect on oral squamous cell carcinoma

作　　者：魏秀峰[1] 高文信[1] 周传香[2] 柳淑杰[1] 蔡研[1] 王家凤[1] 

机构地区：[1]吉林大学口腔医院口腔黏膜病科,吉林长春130041 [2]北京大学口腔医学院口腔医院口腔病理研究室,北京100081

出　　处：《吉林大学学报（医学版）》2009年第5期821-825,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(20050405-1);吉林大学创新基金资助课题(419070200084)

摘　　要：目的:观察转染PTEN的鳞癌细胞的增殖情况及PTEN在口腔白斑及鳞癌中的表达,探讨PTEN基因在口腔鳞癌发展过程中的作用。方法:采用RT-PCR法从正常组织中扩增PTEN基因,构建真核表达载体pcDNA-PTEN,采用脂质体将pcDNA-PTEN转染人舌鳞癌(Tca8113)细胞系,MTT法观察转染前后细胞的增殖情况,流式细胞术检测PTEN基因转染后细胞各周期细胞所占比例。免疫组织化学技术检测口腔白斑(25例)和口腔鳞癌组织(32例)中PTEN蛋白表达率。结果:成功构建pcDNA-PTEN载体,并获得阳性转染细胞。与空载体组相比,转染pcDNA-PTEN组的舌癌细胞系Tca8113细胞的生长抑制率具有显著性差异(P<0.01);与空载体组比较,转染PTEN基因的Tca8113细胞G0-G1期细胞数增多(P<0.01),S期细胞减少(P<0.01);PTEN在口腔鳞癌中表达减少或缺失,且与组织分化程度明显相关(P<0.05)。结论:PTEN的表达可抑制舌鳞癌细胞的生长;PETN在舌鳞癌发展过程中发挥抑癌作用。Objective To explore the role of PTEN in the development of oral squamous cell carcinoma（OSCC） by investigating the effect of PTEN on the proliferation of oral squamous carcinoma cells and its expression in oral leukoplakia（OLK） and OSCC.Methods The eukaryotic expression vector pcDNA-PTEN was constructed successfully,and then was transfected into squamous carcinoma cell line Tca8113.After the stablely transfected Tca8113 cell lines were established,MTT and flow cytometry were used to detect the growth and apoptosis of Tca8113 cells,respectively.The endogenous protein expression level of PTEN was determined by immunohistochemical method in 25 cases of oral leukoplakia and 32 cases of OSCC.Results The over-expression of PTEN gene in pcDNA-PTEN group obviously inhibited the growth and induced the apoptosis of Tca8113 cells compared with control group（P〈0.01）.Moreover,compared with control group,the cells at G0/G1 phase were significantly increased（P〈0.05）,but decreased at S phase（P〈0.05）.In OSCC,the expression of PTEN demonstrated absent or less than normal tissues and the expression level of PTEN had a significantly positive correlation with the malignancy of OSCC（P〈0.05）.Conclusion As a tumor suppressor gene,the expression of PTEN could suppress the growth of OSCC cell line in vitro.PTEN might play an important role in the development of OSCC.

关 键 词：PTEN基因 白斑 口腔 肿瘤 鳞状细胞 

分 类 号：R781.5[医药卫生—口腔医学]