山葡萄籽提取物中总多酚的含量测定  

Determination of totol polyphenols in Vitis amurensis seed extract

作　　者：贾荣[1] 倪海镜[1] 赵春芳[2] 戴维群[1] 

机构地区：[1]吉林大学中日联谊医院药剂科,吉林长春130033 [2]吉林大学药学院药物分析教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2009年第5期877-879,共3页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(200302-05)

摘　　要：目的：建立山葡萄籽提取物中总多酚含量测定的方法。方法：以没食子酸为对照品,采用Folin-Ciocalteus比色法最适宜条件对山葡萄籽提取物中总多酚含量进行测定。结果：没食子酸对照品在浓度为0.002-0.010 g·L^-1范围内,与吸光度成良好的线性关系（Y=86.243X＋0.007,r=0.9996）;精密度试验RSD为0.14%;稳定性实验RSD为1.77%;重复性实验RSD为1.44%;平均回收率为102.00%,RSD为1.37%。结论：该法灵敏度高,重复性好,简便快捷。Objective To establish the determination method of total polyphenols in vitis amurensis seed extract. Methods Using gallic acid as reference substance, the content of total polyphenols in Vitis amurensis seed extract was determined by the optimum conditions of Folin-Ciocalteu chromatometry. Results There was a good linearity between gallic acid concentration and absorbance in the range 0. 002--0. 010 g·L^-1（Y=86. 243X＋0. 007, r= 0. 9996）; the precision RSD was 0. 14%; the stability RSD was 1.77%; the repeatability RSD was 1.44%; the average recovery rate was 102.00~ （RSD= 1.37%）. Conclusion This method is simple, fast and has high sensitivity and good reproducibility.

关 键 词：山葡萄籽 总多酚 没食子酸 比色法 

分 类 号：R284.2[医药卫生—中药学]