海兔素对人乳腺癌细胞增殖及血管内皮生长因子表达的影响  

EFFECT OF APLYSIN ON PROLIFERATION AND VASCULAR ENDOTHELIAL GROWTH FACTOR EXPRESSION IN HUMAN BREAST CANCER CELL LINE

作　　者：贺娟[1] 梁惠[2] 韩磊[1] 马爱国[2] 孙永叶[2] 

机构地区：[1]青岛大学医学院附属医院营养科,青岛266003 [2]青岛大学医学院营养研究所,青岛266021

出　　处：《营养学报》2009年第5期482-485,共4页Acta Nutrimenta Sinica

基　　金：青岛市科学技术局资助项目(No.04-2-HH-75)

摘　　要：目的观察海兔素(aplysin)对人乳腺癌细胞系MCF-7细胞增殖和血管内皮生长因子(VEGF)表达的抑制作用,以及对二甲基苯蒽(DMBA)诱导乳腺癌大鼠肿瘤组织中VEGF表达的抑制作用。方法用MTT法和ELISA法分别检测海兔素对MCF-7细胞增殖及VEGF表达的影响。用免疫组织化学法检测海兔素对DMBA诱导的大鼠乳腺癌组织中VEGF的影响。结果海兔素体外对MCF-7细胞有明显的增殖抑制作用,并伴随VEGF表达水平下降。体内可有效抑制大鼠乳腺癌组织中VEGF的表达,且与海兔素浓度有关。结论海兔素体外对MCF-7细胞具有明显的细胞毒作用并能抑制其VEGF的表达,体内可有效抑制乳腺癌组织中VEGF的表达。Objective To assess the effects of aplysin on proliferation inhibition and vascular endothelial growth factor （VEGF） level in human breast carcinoma （MCF-7） cells in vitro and to investigate the VEGF level of the brest cancer tissue induced by DMBA in rats. Method After MCF-7 cells were treated with aplysin from 10 to 40μg/ml for 48h and 96h, cell viability of proliferation was assessed by MTT assay and VEGF level was assessed by ELISA. Seventy-five female Wistar rats were randomly divided into five groups and were daily given 20mg/kg·bw（A group）,40mg/kg· bw （B group）, 80mg/kg·bw （C group） aplysin, and salad oil for both D and E groups. The rats in A, B, C and D groups were induced breast cancer by DMBA, a carcinogen, and those in E group were free from DMBA during 16 w treatment. VEGF level of the brest cancer tissue was assessed by immunohistochemistry. Results Within the concentration of 10- 40 μg/ml, aplysin strongly inhibited proliferation of MCF-7 cells, accompanied by decrease in VEGF level. Aplysin decreased expression of VEGF in DMBA-induced breast cancer tissue of rats. Conclusion Aplysin could inhibit breast cancer cells with cytotoxicity and VEGF expression. Aplysin could decrease the expression of VEGF in breast cancer tissue induced by DMBA in rats.

关 键 词：海兔素 乳腺癌 血管内皮生长因子 MCF-7细胞 

分 类 号：R737.9[医药卫生—肿瘤] R735.2[医药卫生—临床医学]