人乳腺癌组织中Wnt/β-catenin信号传导通路的异常表达与Her2阳性表达间的关系  

Relationship between ectopic expression of Wnt/β-catenin signaling pathway and Her2 overexpression in human breast carcinoma

作　　者：王珂[1] 任予[1] 何建军[1] 张云锋[1] 陈武科[1] 马清涌[2] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤中心乳腺外科,陕西西安710061 [2]西安交通大学医学院第一附属医院肝胆外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2009年第5期587-591,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：环境与基因相关疾病教育部重点实验室开放基金资助项目(No.KFJJ-2005-05)~~

摘　　要：目的观察人乳腺癌组织中Wnt/β-catenin信号传导通路主要组分E-cadherin、β-catenin、Cyclin D1的异常表达及其关键组分β-catenin的异常表达与Her2阳性表达之间的关系。方法采用免疫组织化学Elivision两步法检测了90例人乳腺癌组织中Her2、E-cadherin、β-catenin以及Cyclin D1的表达情况。结果E-cadherin表达正常组中β-catenin的异常表达率(27.3%)显著低于E-cadherin表达缺失组(75.0%);β-catenin异常表达组中Cyclin D1过表达率(83.3%)显著高于β-catenin正常组(40.7%)。Her2阳性组中β-catenin的异常表达率(73.5%)明显高于Her2阴性组(19.6%)。当Her2的阳性表达与β-catenin的异常表达同时存在时腋窝淋巴结转移的阳性率较高。结论在一些人乳腺癌组织中存在Wnt/β-catenin信号传导通路的异常表达,并且其异常表达与Her2的阳性表达有关,二者的异常表达能够协同促进乳腺癌细胞的淋巴结转移。Objective To investigate ectopic expression of key elements of Wnt /β-catenin signaling pathway, including E-cadherin,β-catenin and Cyclin D1, as well as the relationship between cctopic expression of β-catenin and overexpression of Her2 in human breast carcinoma tissues. Methods Immunohistochemical technique （Elivision） was used to detect the expression of Her2 and three elements of Wnt/β-catenin signaling pathway （6- catenin, E-cadherin, Cyclin D1） in 90 samples of human breast carcinoma. Results In E-cadherin normally- expressed group, the ectopic expression rate of β-catenin （27.3%） was significantly lower than that （75.0%） in E-cadherin negative breast carcinoma; in β-catenin ectopic expression group, overexpression rate of Cyclin D1 （83.3%） was significantly higher than that （40.7%） in the other group. In Her2 overexpressed-breast carcinoma tissue, the ectopic expression rate of β-catenin （73.5 % ） was significantly higher than that （19.6 % ） in Her2 negative breast carcinoma. Her2 ovcrcxpression could coexist with ectopic expression of Wnt/β-catenin in promoting axillary lymph node metastasis in human breast carcinoma. Conclusion Ectopic expression of Wnt/β-catenin signaling pathway does exist in some human breast carcinoma tissues, and the abnormal expression correlates with overexpression of Her2. Furthermore, the instantaneous activation of them could present synergism in promoting metastasis of axillary lymph nodes.

关 键 词：人乳腺癌 Her2 WNT信号传导通路 E-cadherin β-catenin CYCLIN D1 

分 类 号：R737.9[医药卫生—肿瘤]