复合酶法提取野木瓜汁的工艺研究  

Study on the Technology of Extracting Chaenomeles cathayensis Juice by Compound Enzymolysis Method

作　　者：吴国卿[1,2] 王文平[1] 陈燕[2] 段娟娟[2] 顾蓉蓉[2] 

机构地区：[1]贵州省发酵工程与生物制药重点实验室,贵州贵阳550003 [2]贵州大学蔡家关校区化学与化工学院,贵州贵阳550003

出　　处：《安徽农业科学》2009年第31期15421-15423,共3页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技厅工业攻关项目(黔科合GY[2009]3052);贵州大学引进人才科研项目(贵大人基合字(2008)017号)

摘　　要：[目的]研究复合酶法提取野木瓜汁的工艺。[方法]以野木瓜为原料,采用复合酶法提取野木瓜汁。[结果]确定了果胶酶与纤维素酶的最佳添加比例为1∶6。复合酶提取野木瓜汁的最佳酶解工艺条件为:复合酶添加量1.0%,酶解温度45℃,pH值4.0,酶解时间2.5 h。在此最佳条件下,野木瓜出汁率可达56.7%,比空白样的出汁率13.7%多出了43.0个百分点。[结论]找到了一种提取野木瓜汁的方法。[ Objective ] The research aimed to study the technology of extracting Chaenomeles cathayensis juice by compound enzymolysis method. [ Method] Choosing Chaenomeles cathayensis as raw material ,the juice yield of the Chaenomeles cathayensis was studied by compound enzymolysis method. [ Result ] The resuhs showed that the best proportion of pectinase and cellulase was 1 ： 6. The optimum conditions of extracting the Chaenomeles cathayensis juice by compound enzymolysis method was as follow ：The enzymolysis temperature 45 ℃, the amount of compound enzyme（ petinase：cellulase = 1：6 ） 1.0% ,pH value 4.0, the enzymolysis time 2.5 h. The Chaenomeles cathayensis juice yield could reach 56.7% under this optimum conditions. It was more 43.0 percentage point than the control which reached 13.7% . [ Conclusion ] The study finds a method of extracting Chaenomeles cathayensis juice.

关 键 词：野木瓜 果胶酶 纤维素酶 出汁率 

分 类 号：S688.2[农业科学—观赏园艺]