黄曲霉毒素B_1对肝癌细胞株SMMC-7721的作用研究  

Effects of Aflatoxin B_1 to Human Hepatoma Cell Line SMMC-7721

作　　者：许菊萍[1,2] 赵永福[1] 张水军[1] 吴阳[1] 

机构地区：[1]郑州大学第一附属医院普外科,郑州市450052 [2]焦作市第二人民医院普外科

出　　处：《医药论坛杂志》2009年第20期4-7,共4页Journal of Medical Forum

摘　　要：目的通过用黄曲霉毒素B1(AFB1)处理人肝癌细胞株SMMC-7721研究AFB1对SMMC-7721细胞生长的影响。方法①首先应用96孔板培养细胞,选择适宜的细胞接种密度进行实验。②应用不同浓度的黄曲霉毒素B120g/ml、10g/ml、5g/ml、2g/ml、1.0g/ml、0.1g/ml、0.01g/ml处理人肝癌细胞株SMMC-7721,MTT法绘制细胞生长曲线,观察黄曲霉毒素B1对肝癌细胞株SMMC-7721生长的影响。结果不同浓度的AFB1对肝癌细胞株SMMC-7721的影响不同,AFB120.0g/ml、AFB110.0g/ml、AFB15.0g/ml表现为细胞毒性,呈浓度依赖性;AFB11.0g/ml、AFB10.1g/ml、AFB10.01g/ml对肝癌细胞株SMMC-7721的增殖活性具有促进作用,以AFB11.0ug/ml浓度显著,AFB10.1 g/ml、AFB10.01g/ml作用渐减弱。结论黄曲霉毒素B11.0g/ml具有增强人肝癌细胞株SMMC-7721增殖活性的作用。Objective To investigate the effects of aflatoxin B1 to Human Hepatoma Cell Line SMMC-7721.Methods ① Firstly,selected the best density of inoculating cell by culturing cell.②Applied different concentrations of aflatoxin B1 20g/ml,10g/ml,5g/ml,2g/ml,1.0g/ml,0.1g/ml,0.01g/ml to Human hepatoma cells Line SMMC-7721,and took MTT method to draw cell growth curve,observed the function of aflatoxin on SMMC-7721.Selected the best concentration of AFB1 1.0g/ml as the further experimental intervention factor.Results Different concentration of AFB1 played different effects on hepatoma cells SMMC-7721,the concentration of AFB120g/ml,AFB110g/ml,AFB15g/ml,showed cytotoxicity,on the other hand AFB1 1.0g/ml,AFB1 0.1g/ml,AFB1 0.01g/ml promoted proliferation of hepatoma cells,and AFB1 1.0g/ml roled significantly,the role of AFB1 0.1g/ml,AFB1 0.01g/ml gradulaly diminished.Conclusion Aflatoxin B1 can promote the proliferation of SMMC-7721

关 键 词：人肝癌细胞株SMMC-7721 黄曲霉毒素B1 

分 类 号：R735.7[医药卫生—肿瘤]