人胎盘组织液氨基氮含量检测方法的建立  

Qualitative Method of Amino Nitrogen in Human Placenta Tissue Hydrolysate

作　　者：任跃明[1] 肖林[2] 

机构地区：[1]国家药典委员会,北京100061 [2]中国药品生物制品检定所,北京100050

出　　处：《生物技术通讯》2009年第5期687-688,共2页Letters in Biotechnology

摘　　要：目的:建立检测人胎盘组织液氨基氮含量的方法,为产品质量控制和生产工艺稳定性提供判断手段。方法:加入福尔马林溶液后,以麝香草酚蓝为指示剂,用NaOH滴定液滴定。结果:当福尔马林溶液为中性、NaOH滴定液浓度为0.04mol/L时,甲醛滴定氨基氮的方法可靠。结论:本方法回收率高,操作简便,可以作为检测人胎盘组织液氨基氮含量的方法。Objective： To establish a method for detecting content of amino nitrogen in human placenta tissue hydrolysate, also supply a criterion for product quality control and stability of production. Methods： First, add formaldehyde solution, using thymolsulfonphthalein as indicator and sodium hydroxide as volumetric solution, then titrate. Results： When the formalin solution is neutral and the concentration of titration solution sodium hydroxide is 0.04 mol/L, the method of using formalin to titrate amino nitrogen is credible. Conclusion： It＇s a good method for detecting content of amino nitrogen in human placenta tissue hydrolysate, with high recovery and easy operation.

关 键 词：人胎盘组织液 氨基氮 含量 滴定法 

分 类 号：R927.1[医药卫生—药学]