暗纹东方鲀TTX的微量、快速检测  

Rapid and Microanalytic Method for Determination of Tetrodotoxin by High Performance Liquid Chromatography

作　　者：刘子列[1] 华元渝[1] 钱林峰 罗晶[1] 李勤[1] 

机构地区：[1]南京师范大学生物系,南京210097 [2]江苏省水产局,南京210003

出　　处：《水产养殖》1998年第6期17-19,共3页Journal of Aquaculture

摘　　要：采用高效液相色谱法建立快速、微量检测河毒素方法。使用WaterspicoTagTMFAA色谱柱；以5％乙腈中含0．005N三氟乙酸和0．005N乙酸为流动相；荧光检测波长：激发波长为365nm，发射波长为510nm。该方法在25ng／ml～300ng／ml线性范围内峰面积响应值与其浓度呈良好的线性关系，r＝0．9994。最低检测线为1ng。该方法可简便、快速、微量、准确定性、定量检测河毒素。To develop a rapid and microananlytic method for determination of tetrodotoxin by HPLC. With Waters Pico TagAAFAA column,0.5% MeCN solution containing 0.005 N CF3, COOH and 0.05 N HOAc as mobile phase,fluorometric detector excitation and emission wavelengths, 365 and 510nm. The calibration curves was linear in the range of 25ng/ml for the toxin-fluorescence, r=0. 9994. The lower limit of detection was lng. The method was found to be simple,rapid and accurate for determination of tetrodotoxin.

关 键 词：河豚毒素 河豚 HPLC 

分 类 号：S965.225[农业科学—水产养殖]