海洋异养细菌对小球藻生长和无机营养盐吸收的影响研究  

The effect of Chlorella growth and inorganic nutrients absorption by marine heterotrophic bacteria

作　　者：白洁[1] 易齐涛[1] 李佳霖[1] 

机构地区：[1]海洋环境与生态教育部重点实验室,中国海洋大学环境科学与工程学院,山东青岛266100

出　　处：《海洋科学》2009年第11期6-10,共5页Marine Sciences

基　　金：山东省环保局重点基金项目(2004040-1);中国海洋大学物理海洋教育部重点实验室开放基金项目

摘　　要：从胶州湾海洋异养细菌同小球藻(Chlorella vulgaris)的共存体系中,分离出6株形态特征差异较大的异养细菌,并做变性凝胶梯度电泳(Denature Gel Gradient Electrophoresis,DGGE)分析。其中一株对小球藻的生长有较大的促进作用,而其余菌株则没有明显的效果。6株细菌能在小球藻产生的胞外溶解有机物(Dissolved Organic Materials,DOM)溶液中生长良好,共存细菌对提高菌藻体系对无机营养盐的吸收具有较大的贡献。The consortium of marine heterotrophic bacteria and Chlorella vulgaris was separated and six strains of bacteria were isolated, purified and analyzed by the DNA fingerprint of DGGE （denature gel gradient electrophoresis）. One of the strains was found to promote the growth of the C. vulgaris, while the other five strains had no effects. Meanwhile, all of the separated strains could be observed to grow well on the DOC from the pure culture. Compared with the pure culture of C. vulgaris, the mixed culture of the consortium was observed a more efficiency of removing inorganic nitrogen. Also, the absorption of inorganic nutrients was considerably made by bacteria.

关 键 词：小球藻(Chlorella vulgaris) 异养细菌 共生关系 富营养化 变性凝胶梯度电泳(DGGE) 无机营养盐 

分 类 号：X172[环境科学与工程—环境科学]