一种简捷的生物素含量测定方法  

A Simple Method for Detecting Content of Biotin

作　　者：郑巍振[1] 裘娟萍[1] 赵春田[1] 朱家荣[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310014

出　　处：《食品与发酵工业》2009年第10期135-137,共3页Food and Fermentation Industries

摘　　要：为建立一种快速方便检测生物素含量的方法,采用生物素营养缺陷型菌株产氨短杆菌作为指示菌,研究了管碟法测定生物素含量的方法。研究结果表明,生物素浓度在15-95μg/L,平板指示菌生长圈直径大小与生物素浓度对数值呈一定线性关系,且线性良好,r=0.9933;平均回收率为101.0%,相对标准偏差4.984%-7.573%。In order to establish a convenient and rapid method to detect the content of biotin, the oxford plate assay system used to detect content of biotin was studied in this paper using Corynebacterium ammoniagenes, which is auxotrophic for biotin, as indicator bacteria. The results showed that a better linear relation between the diameter of the flat-panel indicator bacteria growth rings and the biotin concentration logarithm was obtained in the detected concentration range from 15μg/L to 90 μg/L（r = 0. 993 3）. The average recovery rate of this measurement was 101.0% and relative standard deviation（RSD） ranged from 4. 984% to 7. 573%.

关 键 词：生物素 检测 管碟法 

分 类 号：R151[医药卫生—营养与食品卫生学]