p38MAPK在慢性髓细胞白血病信号转导中的作用  

Role of p38 mitogen-activated protein kinase in the signal transduction of chronic myelogenous leukemia

作　　者：尚振川[1] 宋霆婷[2] 孙秉中[3] 张涛[3] 易海[1] 付利[1] 符宇[1] 

机构地区：[1]成都军区总医院血液科,成都610083 [2]青岛市肿瘤医院内二科 [3]第四军医大学西京医院血液科

出　　处：《临床血液学杂志》2009年第6期597-599,共3页Journal of Clinical Hematology

基　　金：国家自然科学基金课题资助(No:39670330;No:39770336)

摘　　要：目的:探讨p38MAPK在慢性髓细胞白血病(CML)信号转导中的作用。方法:用脂质体转染法将bcr3/abl2反义寡核苷酸(ASO)导入K562细胞中,通过细胞凋亡、p38MAPK活性、p38MAPK表达和p38MAPK含量的变化,检测p38MAPK在CML信号转导中的作用。结果:bcr3/abl2ASO作用K562细胞24h后凋亡细胞比率、p38MAPK活性、p38MAPK表达和p38MAPK含量明显增加,分别为对照组的3.6、3.3、2.7和1.8倍,与对照组之间均差异有统计学意义(均P<0.05)。结论:p38MAPK通过介导凋亡信号而在CML信号转导中起重要作用。Objective：To study the role of p38 mitogen-activated protein kinase（p38MAPK） in the signal transduction of chronic myelogenous leukemia（CML）.Method：After Bcr3/abl2 antisense oligodeoxynucleotide（ASO） was introduced into K562 cell line by liposomal transfection,the effect was evaluated by apoptosis,p38MAPK activity,p38MAPK expression and p38MAPK content.Result：After 24 hours treatment by ASO,the rate of apoptotic cells,p38MAPK activity,p38MAPK expression and p38MAPK content were 3.6,3.3,2.7 and 1.8 times higher than that in control groups respectively（P〈0.05）.Conclusion：p38MAPK plays an important role in the signal transduction of CML by directing apoptotic signal.

关 键 词：白血病 慢性 反义寡核苷酸 丝裂原活化的蛋白激酶 信号转导 

分 类 号：R733.72[医药卫生—肿瘤]