牛磺酸对鼠脑缺血再灌注谷氨酸转运体功能的影响  

Influence of taurine on the function of glutamate transporter in rats with injury of cerebral ischemiareperfusion

作　　者：任晓燕[1] 张玉敏[1] 曲忠森[1] 葛宝林[1] 

机构地区：[1]青岛大学医学院病理生理教研室

出　　处：《中华神经科杂志》1998年第4期235-237,共3页Chinese Journal of Neurology

基　　金：国家自然科学基金

摘　　要：目的探讨牛磺酸对鼠脑缺血再灌注（ＩＲ）时皮层、海马、纹状体谷氨酸转运体功能的影响。方法采用３血管夹闭、松夹制作鼠ＩＲ模型。利用脑组织突触膜颗粒对３ＨＬ谷氨酸摄入量的测定及分光光度法观察谷氨酸转运体、丙二醛（ＭＤＡ）含量及超氧化物歧化酶（ＳＯＤ）活性的变化。结果与对照组相比，ＩＲ组皮层、海马及纹状体谷氨酸转运体的功能及ＳＯＤ活性低（Ｐ＜０．０５，０．０１），ＭＤＡ含量高（Ｐ＜０．０５，０．０１）。与ＩＲ组相比，牛磺酸组鼠脑皮层、海马及纹状体的功能及ＳＯＤ活性高（Ｐ＜０．０５，０．０１），ＭＤＡ含量低（Ｐ＜０．０５，Ｐ＜０．０１）。结论牛磺酸可改善ＩＲ后皮层、海马及纹状体谷氨酸转运体的功能。Objective To investigate the effect of taurine on the function of glutamate transporter in rats with injury of cerebral ischemiareperfusion. Methods A model of ischemiareperfusion injury (IR) was performed by clipping three arteries and then by releasing them to reperfuse blood into the ischemic brain in rats. Glutamate transporter functions were studied by means of assay of 3HLglutamate uptake in synaptosomes, and MDA contents and SOD activities in cerebral cortex, hippocampus, striatum by spectrophotometry.Results Glutamate transporter function and SOD activity in IR group were significantly decreased (P<0.05,0.01); content of MDA were significantly increased (P<0.05, 0.01) while compared with control group. Glutamate transporter function and SOD activity in taurine group were significantly increased(P<0.05, 0.01); MDA content was significantly decreased (P<0.05, 0.01) while compared with IR group. Conclusion Taurine can improve glutamate transporter function in IR rats. The mechanism might be related to eliminate free radicals.

关 键 词：脑缺血 再灌注损伤 谷氨酸 牛磺酸 

分 类 号：R743.3[医药卫生—神经病学与精神病学]