响应面法优化金银花多糖超声提取工艺研究  

Optimization of Ultrasonic Extraction for Polysaccharides from Lonicera japonica

作　　者：赵鹏[1] 李稳宏[1] 朱骤海[1] 李冬[1] 高蓉[1] 

机构地区：[1]西北大学化工学院,陕西西安710069

出　　处：《食品科学》2009年第20期151-154,共4页Food Science

摘　　要：为优化金银花多糖的超声提取工艺,在单因素试验的基础上,选取温度、时间以及液料比为自变量,多糖提取率为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对多糖提取率的影响。利用响应面分析方法,模拟得到二次多项式回归方程的预测模型,并确定金银花多糖提取工艺的最佳条件为温度75℃、时间43min、液料比23:1(ml/g)、提取两次。在此条件下,多糖提取率达到3.263%。超声法与传统的回流提取法相比,最大的特点是提取时间明显缩短。In order to optimize ultrasonic extraction of polysaccharides in Lonicerajaponica, a mathematical regression model for predicting extraction yield of polysacchafides as a function of ratio of independent variables, including water/material ratio, extraction temperature and extraction time was established by fitting Box-Benhnken central composite design experimental results and interactive effects of the independent variables on extraction yield of polysaccharides were investigated by response surface methodology based on the analysis of single-factor experiments. The optimal protocol for polysaccharides extraction from Lonicerajaponica was based on twice extraction for 43 min at 75 ℃ with a ratio of water to dry material of 23：1, which yielded an extraction yield of 3.26%. Results indicated that ultrasonic method is characteristic of time-saving compared with other traditional methods.

关 键 词：金银花 超声提取 多糖 工艺条件 响应面分析 

分 类 号：TS272[农业科学—茶叶生产加工]