槐花α-葡萄糖苷酶抑制活性研究  

α-Glucosidase Inhibitory Activity of Sophora japonica L.

作　　者：康文艺[1,2] 宋艳丽[1] 崔维恒[1] 

机构地区：[1]河南大学天然药物研究所,河南开封475004 [2]河南大学中药研究所,河南开封475004

出　　处：《精细化工》2009年第11期1077-1079,1092,共4页Fine Chemicals

基　　金：河南省教育厅青年骨干教师资助计划(教高2008-755);河南大学大学生创新性实验计划(09NB026)~~

摘　　要：用体外α-葡萄糖苷酶抑制模型,对槐花乙酸乙酯、石油醚、正丁醇、丙酮水总提取物进行活性筛选,对提取物浓度与抑制活性关系进行了研究,并对强活性的3种浸膏进行了抑制动力学研究。槐花总提取物(IC50=50.56 mg.L-1)、乙酸乙酯部位(IC50=1.25 mg.L-1)、正丁醇部位(IC50=16.14 mg.L-1)。活性远高于对照Acarbose(IC50=1 081.27 mg.L-1);并全部属于非竞争性抑制类型(Ki值分别为4.53、652.5 mg.L-1和62.38mg.L-1);从乙酸乙酯部位得到活性成分槲皮素(IC50=8.86 mg.L-1)和山奈酚(IC50=73.69 mg.L-1)。结果表明,槐花乙酸乙酯部位可作为降血糖活性部位进行体内研究。该文报道的新颖性已为河南大学图书馆2009年6月24日出具的第CX200906242号《科技查新报告》所证实。In order to investigate the α-glucosidase inhibitory activity of Sophora japonica,the total extract acetone-water extract and those extracts including petroleum ether,ethyl acetate and n-BuOH were screened by α-glucosidase inhibitory model in vitro.The relationship between the inhibitory ratio and the extract concentration was assayed and the inhibitory kinetics was also investigated.The ethyl acetate extract（IC50=1.25 μg·mL^-1）,n-Butanol extract（IC50=16.14 μg·mL^-1） and acetone-water extract（IC50=50.56 μg·mL^-1）showed a strong inhibitory activity,which is higher than that of Acarbose（IC50=1081.27 μg·mL^-1）.All of them the shown noncompetitive type model on α-glucosidase with Ki value of 652.5 μg·mL^-1,62.38 μg·mL^-1 and 4.53 μg·mL^-1.Quercetin（IC50=8.86 μg·mL^-1） and kaempferol（IC50=73.69 μg·mL^-1） were isolated from the ethyl acetate extract.The ethyl acetate extract can be further screened as the active fraction of the decreased postprandial blood glucose in vivo.The novelty of this work has been confirmed in the Technology Novelty Search Report No.CX200906242 provided by the library of Henan University on June 24,2009.

关 键 词：槐花 Α-葡萄糖苷酶 抑制活性 抑制类型 

分 类 号：R284.1[医药卫生—中药学] Q946.91[医药卫生—中医学]