人参皂苷单体Rb1及Rg1对白血病细胞K562增殖的影响  

Effects of ginsenoside Rb1,Rg1 on proliferation of leukemia K562 cells

作　　者：王红宁[1] 左国伟[2] 李春莉[3] 官涛[4] 姜蓉[1] 陈地龙[1] 罗春燕[1] 胡晓舒[1] 王建伟[1] 

机构地区：[1]重庆医科大学组胚教研室干细胞与组织工程研究室眼科实验室,重庆市400016 [2]重庆医科大学临床检验诊断学省部共建教育部重点实验室,重庆市400016 [3]重庆医科大学生命科学院,重庆市400016 [4]重庆医科大学生理教研室,重庆市400016

出　　处：《中国组织工程研究与临床康复》2009年第40期7829-7832,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：重庆市科委基金课题资助(2007BB5304)~~

摘　　要：背景:国内外研究发现人参皂苷及其单体在抑制白血病细胞增殖、增加化疗药物的敏感性和逆转耐药等方面均有疗效,目前主要集中于对实体瘤和急性白血病的研究,涉及慢性白血病的报道非常少。目的:探讨人参皂苷单体Rb1,Rg1对人慢性粒细胞白血病细胞K562增殖的影响。设计、时间及地点:细胞学体外观察,于2008-03/2009-03在重庆医科大学干细胞与组织工程研究室和眼科实验室完成。材料:K562细胞由重庆医科大学临床检验系提供。纯度为98.6%的人参皂苷单体Rb1,Rg1由南京艾斯替么中药研究所提供。方法:取对数生长期的K562细胞,调整密度为7×108L-1,空白对照组予以常规培养;人参皂苷单体Rb1组分别加入20,40,80,160μmol/L的Rb1;人参皂苷单体Rg1组分别加入5,10,20,40,80μmol/L的Rg1。主要观察指标:MTT比色法、锥虫蓝拒染法检测K562细胞增殖情况,流式细胞仪测定细胞周期分布的变化。结果:人参皂苷单体Rb1,Rg1在体外对K562细胞增殖均有明显的抑制作用,在20～160μmol/LRb1和5～20μmol/LRg1浓度范围内,其抑制作用呈浓度依赖性,且均在作用48h时抑制率达高峰。与空白对照组比较,体外培养48h后160μmol/LRb1组细胞周期分布无变化;20μmol/LRg1组S期细胞比例明显增加(P<0.05),G1期细胞比例明显下降(P<0.05),且160μmol/LRb1组、20μmol/LRg1组均未出现"亚二倍体"峰。结论:人参皂苷单体Rb1,Rg1均可抑制K562细胞增殖,Rg1增殖抑制作用可能是通过将K562细胞阻滞于S期而实现的,而Rb1的增殖抑制作用与细胞周期的关系有待进一步分析。BACKGROUND：Domestic and international studies demonstrated that ginsenoside and its monomers inhabit leukemia cells proliferation and increase sensitivity and reversing drug resistance of chemotherapeutics. However,most research regarding ginsenoside is focus on solid tumor and acute leukemia,and the reports concerning chronic leukemia are few. OBJECTIVE：To investigate the effect of ginsenoside Rb1,Rg1 on proliferation of chronic myelocytic leukemia K562 cells. DESIGN,TIME AND SETTING：An in vitro cytology observation. The experiment was performed at the Department of Histology and Embryology,Laboratory of Stem Cell and Tissue Engineering,Laboratory of Ophthalmology of Chongqing Medical University from March 2008 to March 2009. MATERIALS：K562 cells were supported by Department of Clinical Laboratory,Chongqing Medical University. ginsenoside Rb1,Rg1 with purity of 98.6% was provided by Estima Institute of Chinese Medicine. METHODS：The density of K562 cells with logarithmic phase were regulated to 7×108/L and incubated with 20,40,80,160 μmol/L ginsenoside Rb1 or 5,10,20,40,80 μmol/L ginsenoside Rg1,respectively. The conventional culture was performed in the blank control group. MAIN OUTCOME MEASURES：The proliferation of K562 cells was examined by MTT and trypan blue staining;meantime,the changes of cell cycle were determined by flow cytometry. RESULTS：Ginsenoside Rb1,Rg1 inhibited K562 cells proliferation significantly in vitro,which exhibited a dose-dependent manner at range of 20-160 μmol/L Rb1 and 5-20 μmol/L Rg1,and reached peak at hour 48. Compared to the blank control group,there was no cell cycle change in the 160 μmol/L Rb1 group at hour 48 after culture. However,S phase cells in the 20 μmol/L Rg1 group were dramatically increased（P 0.05） ,G1 phase cells were decreased（P 0.05） ;in addition,no ＂subdiploid＂ peak appeared in the 160 μmol/L Rb1 and 20 μmol/L Rg1 groups. CONCLUSION：Ginsenoside Rb1,Rg1 can inhibit the proliferation of K562 cells in vitro. The inhibitory mechani

关 键 词：人参皂苷单体 RB1 RG1 K562细胞 细胞周期 增殖抑制 

分 类 号：R394.2[医药卫生—医学遗传学]