Antifi brotic effects of green tea on in vitro and in vivo models of liver fibrosis  

作　　者：Hye Kyung Kim Taik-Hoon Yang Hong-Yon Cho 

机构地区：[1]Department of Food and Biotechnology, Hanseo University, Seosan, Chungnam 356-706, South Korea [2]Department of Food and Bio-technology, College of Science and Technology, Korea University, Jochiwon, Chungnam 339-700, South Korea

出　　处：《World Journal of Gastroenterology》2009年第41期5200-5205,共6页世界胃肠病学杂志（英文版）

基　　金：Supported by A Korea University Grant

摘　　要：AIM: To examine the protective effect of green tea extract (GT) on hepatic fi brosis in vitro and in vivo in dimethylnitrosamine (DMN)-induced rats.METHODS: HSC-T6, a rat hepatic stellate cell line, was used as an in vitro assay system. Cell proliferation, collagen content, and type 1 collagen expression were examined in activated HSC-T6 cells. Collagen was determined by estimating the hydroxyproline content. In rats with DMN-induced hepatic fi brosis, serum aspartate aminotransferase and alanine aminotransferase concentrations, liver hydroxyproline and lipid peroxides were determined. Pathologic changes were examined by hematoxylin & eosin staining.RESULTS: GT administration prevented the development of hepatic fibrosis in the rat model of DMN-induced liver fi brosis. These results were confi rmed both by liver histology and by quantitative measurement of hepatic hydroxyproline content, a marker of liver collagen deposition. Accordingly, inhibition of proliferation, reduced collagen deposition, and type 1 collagen expression were observed in activated HSC-T6 cells following GT treatment. These results imply that GT reduced the proliferation of activated HSC and down regulated the collagen content and expression of collagen type 1, thereby ameliorating hepatic fibrosis.CONCLUSION: This study demonstrates that greentea administration can effectively improve liver fibrosis caused by DMN, and may be used as a therapeutic option and preventive measure against hepatic f ibrosis.AIM： To examine the protective effect of green tea extract （GT） on hepatic fibrosis in vitro and in vivo in dimethylnitrosamine（DMN）-induced rats. METHODS： HSC-T6, a rat hepatic stellate cell line, was used as an in vitro assay system. Cell proliferation, collagen content, and type 1 collagen expression were examined in activated HSC-T6 cells. Collagen was determined by estimating the hydroxyproline content. In rats with DMN-induced hepatic fibrosis, serum as- partate aminotransferase and alanine aminotransferase concentrations, liver hydroxyproline and lipid peroxides were determined. Pathologic changes were examined by hematoxylin ＆ eosin staining. RESULTS： GT administration prevented the development of hepatic fibrosis in the rat model of DMN-induced liver fibrosis. These results were confirmed both by liver histology and by quantitative measurement of hepatic hydroxyproline content, a marker of liver collagen deposition. Accordingly, inhibition of proliferation, reduced collagen deposition, and type 1 collagen expression were observed in activated HSC-T6 cells following GT treatment. These results imply that GT reduced the proliferation of activated HSC and down regulated the collagen content and expression of collagen type 1, thereby ameliorating hepatic fibrosis. CONCLUSION： This study demonstrates that green tea administration can effectively improve liver fibrosis caused by DMN, and may be used as a therapeutic option and preventive measure against hepatic fibrosis.

关 键 词：DIMETHYLNITROSAMINE Green tea extract HSC-T6 cell Liver fibrosis Rat model Type 1 collagen 

分 类 号：R575.2[医药卫生—消化系统]