甘氨脱氧胆酸盐诱导人正常肝细胞株HL-7702凋亡过程中caspase-3,8活性与mRNA表达水平变化  

Changes in caspase-3,8 activity and mRNA expression of normal human hepato-cyte HL-7702 cell in the process of apoptosis induced by glycodeoxycholate

作　　者：传良敏[1] 黄文芳[1] 饶绍琴[1] 洪华[1] 邓君[1] 杨永长[1] 

机构地区：[1]四川省医学科学院.四川省人民医院检验科,成都610072

出　　处：《重庆医科大学学报》2009年第10期1338-1340,共3页Journal of Chongqing Medical University

摘　　要：目的:观察甘氨脱氧胆酸盐(Glycodeoxycholate,GCDC)诱导人正常肝细胞株HL-7702凋亡过程中caspase-3,8活性与mRNA表达水平的变化,探讨GCDC诱导肝细胞凋亡的机制。方法:体外培养HL-7702细胞,不同浓度的GCDC为处理因素,用AnnexinⅤ-FITC/PI双染色法结合流式细胞技术仪检测细胞凋亡率,比色法测定caspase-3,8活性,RT-PCR检测caspase-3,8mRNA表达水平。结果:100～250μmol/L的GCDC处理HL-7702细胞24 h后,其细胞凋亡率、caspase-3,8活性与mRNA表达水平明显升高,并与GCDC呈浓度依赖性。结论:caspase-3,8参与GCDC诱导HL-7702细胞凋亡的调控,GCDC通过活化caspase-8,并进一步活化caspase-3从而诱导肝细胞凋亡。Objective：To investigate the apoptosis and changes of caspase-3,8 activity and mRNA expression induced by glycodeoxycholate（GCDC） in cultured normal human hepatocyte HL-7702 cell in vitro and to explore the possible mechanism of hepatocyte apoptosis.Methods：HL-7702 cells were cultured with GCDC,the final concentration of which were 100、150、200 and 250 μmol/L respectively.The apoptosis ratio of HL-7702 cell was determined by AnnexinⅤ-FITC/PI double staining.Colorimetric method was used to measure caspase-3,8 activity .The expression of caspase-3,8 mRNA was determined by RT-PCR. Results： When hepatocytes were incubated with GCDC for 24 hours , the apoptotic ratio of hepatocyte , caspase-3,8 activity and mRNA expression of HL-7702 cells increased remarkably and were concentra- tion-dependent on GCDC. Conclusion： The caspase-3,8 were concerned with the hepatocyte apoptosis induced by GCDC.The mechanism might be related to the upregulation of caspase-8 activity which subsequently transforms caspase-3 into its active form.

关 键 词：甘氨脱氧胆酸盐 细胞凋亡 CASPASE 

分 类 号：R575[医药卫生—消化系统]