儿童喉乳头状瘤组织中Survivin及Caspase-3蛋白表达及相关性研究  

Correlation of the expression of survivin and caspase-3 proteins in juvenile laryngeal papilloma

作　　者：汪武[1] 周远大[2] 何海霞[2] 

机构地区：[1]重庆医科大学附属儿童医院耳鼻喉科,重庆400014 [2]重庆医科大学临床学院临床药理研究室,重庆400016

出　　处：《细胞与分子免疫学杂志》2009年第11期1034-1035,共2页Chinese Journal of Cellular and Molecular Immunology

基　　金：重庆市自然科学基金重点课题(CSTC2006EB5030)

摘　　要：目的:研究儿童喉乳头状瘤中survivin及caspase-3蛋白的表达及其相互关系。方法:应用免疫组化方法对儿童喉乳头状瘤组织、儿童声带小结组织及正常的喉黏膜组织中survivin及caspase-3的表达进行检测并进行相关性分析。结果:42例儿童喉乳头状瘤组织中survivin阳性表达率为57.14%,明显高于儿童声带小结组(P<0.01)和正常黏膜组(P<0.01);42例喉乳头状瘤组织中caspase-3阳性表达率为26.19%,显著高于儿童声带小结组和正常喉黏膜组(P<0.01);Spearman相关性分析显示,儿童喉乳头状瘤中survivin与caspase-3的表达呈显著负相关(r=-0.682,P<0.01)。结论:Survivin的高表达和caspase-3的低表达可能在儿童喉乳头状瘤发生发展过程中起着重要作用。AIM： To investigate correlation between the expression of survivin and caspase-3 proteins in juvenile laryngeal papilloma. METHODS： The expression of survivin and caspase-3 proteins were detected with immunohistochemial method in 43 cases of juvenile laryngeal papilloma, 25 vocal nodules and 25 normal laryngeal mucosa. RESULTS： The positive rates of survivin protein in juvenile laryngeal papilloma were 57. 14% and higher than that in voeal nodules （ P 〈 0.01 ） and the normal laryngeal moeusa （P〈0.01）. And the Caspase-3 protein positive rate was 26.19% in juvenile laryngeal papilloma and lower than that in voeal nodules and the normal laryngeal mucosa （P 〈 0.01 ）. There was a significant negative correlation between the expression of survivin and caspase-3 in juvenile laryngeal papilloma. CONCLUSION： The abnormal expression of survivin and caspase-3 may play important role in the pathogenesis of juvenile laryngeal papilloma.

关 键 词：喉乳头状瘤 CASPASE SURVIVIN 细胞凋亡 

分 类 号：R739.6[医药卫生—肿瘤]