量子点标记技术检测人晶状体缝隙连接蛋白表达水平  

Expression of Connexin Cx50 in Lenses Detected by the Quantum Dot Labeling Technique

作　　者：严明[1] 周新[2] 陈永梅[2] 赵蔚[3] 严锋锋[4] 

机构地区：[1]武汉大学中南医院眼科,湖北武汉430071 [2]武汉大学中南医院检验科,湖北武汉430071 [3]武汉大学化学与分子科学学院,湖北武汉430072 [4]中国地质大学计划生育办公室,湖北武汉430074

出　　处：《武汉大学学报（医学版）》2009年第6期748-751,I0003,共5页Medical Journal of Wuhan University

基　　金：湖北省自然科学基金资助项目(编号:2008CDB114)

摘　　要：目的:通过建立自制量子点荧光免疫组织化学技术,对人晶状体缝隙连接蛋白(Connexin,Cx)50表达水平进行检测。方法:正常人和先天性白内障患者的晶状体制备石蜡切片,将羧基修饰的量子点和鼠抗兔的二抗相结合,分别采用链霉卵白素-过氧化物酶连结(SP)法和量子点偶联的二抗SP法对正常人和先天性白内障患者的晶状体细胞Cx50蛋白表达水平进行检测。结果:先天性白内障患者晶状体细胞Cx50蛋白表达水平高于正常对照组,量子点偶联的二抗SP法与常规SP法相比,具有敏感性高的优点。结论:量子点荧光标记二抗在检测组织蛋白表达水平中具有一定优势,值得推广。Objective：To establish a novel fluorescence immunostaining assay with quantum dot （QDs） to detect the expression level of Cx50 protein in human lenses. Methods：The lenses tissues from the patients with congenital cataract and from normal controls were prepared as slides. The carboxyl-modified QDs were linked to the goat anti-rabbit IgG. The Cx50 expression levels in the lenese tissue were determined by traditional SP method and improved SP method with QDs-linked-antibody. Results：The expression level of Cx50 protein in patients was higher than that in the control. The improved SP method with QDs-linked-antibody was more sensitive than the SP method. Conclusion：QDs-linked-antibody has advantage in the investigation of protein expression level in tissues for further clinical application.

关 键 词：白内障 量子点 缝隙连接蛋白50 

分 类 号：R776.1[医药卫生—眼科]