蚯蚓堆制处理对不同物料中4种酶活性的影响  

Four Enzyme Activities from Different Feedstocks after Treatment with Eisenia fetida

作　　者：周颖[1] 陈泽光[1] 宦霞娟[1] 薛鹏[1] 徐伟佳[1] 马雄[1] 陈玉林[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《西北农业学报》2009年第6期66-69,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西重大科技创新专项;优质高产良种奶牛选育及标准化生产技术集成与示范[2005ZKC(二)07-01]

摘　　要：旨在研究蚯蚓堆置处理作用对纤维素、半纤维素、木质素降解的影响。采用室内接种的方法,用不同C/N的玉米秸秆与纯牛粪混合物为物料饲养赤子爱胜蚓(Eisenia foetida),以成蚓与幼蚓总数和物料中纤维素酶、木聚糖酶、过氧化物酶和多酚氧化酶的酶活性为检测指标。结果表明,接种蚯蚓的试验组比未接种蚯蚓的对照组能显著增强物料中4种酶的活性;接种蚯蚓的物料C/N为25时,成蚓与幼蚓总数、纤维素酶、过氧化物酶和多酚氧化酶的酶活性较其他组高,3种酶活性随时间逐渐增强;C/N为30时,木聚糖酶活性较其他组高,并随时间逐渐减弱。蚯蚓能在堆制处理过程中加速纤维素、半纤维素和木质素的降解;当C/N=25时,蚯蚓繁殖较好,比较有利于纤维素、半纤维素、木质素降解。To assess the role of earthworms in cellulose,hemicellulose and lignin decomposition,we carried out experiments in which the different C/N mixtures of cattle manure and corn stock were treated in laboratory with Eisenia foetida,Taking the population of earthworms and the activity of Cellulase,Xylanase,Peroxydase（POD）and Polyphenoloxidase（PPO）as indices.It was found that the presence of earthworms stimulated the activity of enzymes;Eisenia foetida showed the best reproduction and the highest activity of Cellulase,POD,and PPO at C /N=25,significantly increasing with periods,However,Xylanase showed the highest activity at C/N=30,decreasing with periods.Eisenia foetida can trigger the decomposition of cellulose,hemicellulose and lignin.The optimal C/N condition for the reproduction of Eisenia foetida,and the decomposition of cellulose,hemicellulose and lignin was 25.

关 键 词：蚯蚓堆置处理 纤维素酶 木聚糖酶 过氧化物酶 多酚氧化酶 

分 类 号：S141.4[农业科学—肥料学]