培养条件对Trametes versicolor SYBC L3固态发酵产漆酶的影响  

Effect of Culture Condition on Laccase Production of Trametes versicolor SYBC L3 by Using Solid State Fermentation

作　　者：王欣[1] 蔡宇杰[1] 廖祥儒[1] 唐蕾[1] 刘家扬[1] 张峰[1] 张大兵[2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江苏汉邦科技有限公司,淮安223001

出　　处：《西北农业学报》2009年第6期174-179,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：长江学者和创新团队发展计划资助项目(IRT0532)

摘　　要：对菌株Trametes versicolor SYBC L3产漆酶固态发酵培养基进行优化,确定该菌适宜的发酵条件为豆粕和木屑比例为1∶1,最适碳源是麦芽糖1.0%(w/w),最佳氮源是硝酸钠2.5%(w/w),硫酸铜浓度为0.02%(w/w)。在250 mL三角瓶中装样40 g,含水量为70%(w/w),提取最适pH为6.5,酶曲最适浸泡时间为4 h时,优化后菌株Trametes versicolor SYBC L3固态发酵所产漆酶酶活可达91.057U/g干曲。The laccase producing medium of Trametes versicolor SYBC L3 by using solid-state fermentation was optimized.The results showed that the optimal soybean meal and sawdust mixed ratio of 1∶1.The optimal carbon source is Maltose 1.0 %（w/w）.The optimal nitrogen is Sodium nitrate 2.5%（w/w）.The concentration of copper sulfate CuSO4 is 0.02%（w/w）.The optimized solid-state fermentation condition was 40 g solid-state medium in 250 mL Erlenmeyer flasks.The optimal medial moisture content is 70%（w/w）.The optimized pH value of the buffer for crude enzyme extract was 6.5.The optimized time for laccase extract was 4 h.After be optimized,the laccase of Trametes versicolor SYBC L3 by using solid-state fermentation

关 键 词：白腐真菌 漆酶 固态发酵 

分 类 号：Q93[生物学—微生物学]