加速溶剂萃取-高效液相色谱-串联质谱法同时测定减肥保健食品中11种食欲抑制剂  

Simultaneous Determination of 11 Appetite Suppressants in Weight-loss Functional Foods by Accelerated Solvent Extraction-High Performance Liquid Chromatography Tandem Mass Spectrometry

作　　者：马微[1,2] 彭涛[3] 朱明达[1] 李晓娟[3] 马强[3] 陈冬冬[3] 李立[3] 国伟[3] 唐英章[3] 

机构地区：[1]中国农业大学,北京100083 [2]东宁出入境检验检疫局,东宁157200 [3]中国检验检疫科学研究院,北京100123

出　　处：《分析化学》2009年第11期1583-1589,共7页Chinese Journal of Analytical Chemistry

基　　金：国家科技支撑计划农业领域课题基金(No.2006BAD27B02)资助项目

摘　　要：建立了测定减肥保健食品中11种非法添加食欲抑制剂(芬氟拉明、苯丙醇胺、西布曲明、舍曲林、利莫那班、安非他酮、西酞普兰、氟西汀、苯氟雷司、托吡酯、唑尼沙胺)的高效液相色谱-串联质谱分析方法。不同类型的减肥保健食品经加速溶剂萃取后,以Waters AtlantisT3柱(150mm×2.1mm,3μm)分离,采用HPLC-MS/MS电喷雾电离,多反应监测模式检测,以保留时间和子离子比定性,外标法定量。11种食欲抑制剂的检出限为0.05～4.0mg/kg;在低、中、高3个添加水平范围内的平均回收率为78.3%～103.6%;日内精密度均小于12%,日间精密度均小于15%。本方法分析速度快、灵敏度高、重现性好,可用于不同减肥保健食品中非法添加食欲抑制剂的检测。An analytical method based on high performance liquid chromatography tandem mass spectrometry has been developed for the determination of 11 appetite suppressants （fenfluramine, phenylpropanolamine, sibutramine, sertraline, rimonabant, bupropion, citalopram, fluoxetine, benfluorex, topiramate, zonisamide）. Various weight-loss functional foods were extracted under accelerated solvent extraction and separated on Waters Atlanti T3（ 150 mm ×2.1 mm, 3 μm） column. The analysis was carried out on HPLC-MS/MS by electrospray ionization using multiple reaction monitoring. Identification was achieved by 1he retention time and the ion ratio, quantification was done by the external standard curves. The limits of detection for the appetite suppressants were 0.05 - 4.0 mg/kg. The mean recoveries at the three spiked levels （ low, middle, high） were 78.3% -103.6%, with the intra-day precision less than 12% and the inter-day precision less than 15%. The method is reliable, sensitive, reproducible, and adapts to the determination of the appetite suppressants in different weight-loss functional foods.

关 键 词：高效液相色谱-串联质谱 加速溶剂萃取 食欲抑制剂 质谱裂解途径 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]