Caspase-3在大鼠齿状回生后发育过程中的作用研究  

Research on the role of caspase-3 in rat dentate gyrus during postnatal development

作　　者：张雪娟[1] 常丽荣[1] 宋一志[1] 陆涛[1] 高秀来[1] 武艳[1] 

机构地区：[1]首都医科大学解剖学教研室,北京100069

出　　处：《神经解剖学杂志》2009年第6期611-615,共5页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30600165);北京市科技新星计划(2006B61);北京市优秀人才培养(20071D0501800252);2006年度留学人员科技活动择优(北京市重点项目)资助项目

摘　　要：为探讨在大鼠海马齿状回(DG)生后发育过程中,活化的caspase-3在细胞增殖中的作用,本研究采用双重免疫荧光染色方法检测活化的caspase-3与Ki-67的表达情况及其相互关系。结果显示:在DG生后发育过程中,活化的caspase-3与Ki-67的表达无相关性,但明显存在小部分共表达的颗粒细胞。研究结果提示,在终生都有自我更新能力的DG中,活化的caspase-3在颗粒细胞增殖中可能存在重要的非凋亡作用。To investigate the effect of active easpase-3 on cell proliferation, dual immunofiuoreseent staining was applied to observe the expression and relationship between active caspase-3 and Ki-57 in the dentate gyrus （DG） of rat hippocampus during postnatal development. The results showed that the expression of active caspase-3 and Ki-67 had no statistical correlation in DG during postnatal development. However, there were a minority of colocalized granular cells evidently. These results suggest that active caspase-3 may exert a significant non-apoptotic function in proliferating granular cells of DG undergoing self-renewal throughout life.

关 键 词：CASPASE-3 KI-67抗原 齿状回 大鼠 生长发育 免疫组织化学 

分 类 号：R741[医药卫生—神经病学与精神病学]