Notch1在食管鳞癌中的表达及其与毛细血管新生的关系  

Notch1 expression in esophageal squamous cell carcinoma and its relation with microvascular angiogenesis

作　　者：苏春华[1] 何裕隆[2] 陈振光[1] 雷艺炎[1] 邹健勇[1] 钟佛添[1] 罗红鹤[1] 

机构地区：[1]中山大学附属第一医院胸外科,广东广州510080 [2]中山大学附属第一医院胃肠外科,广东广州510080

出　　处：《南方医科大学学报》2009年第11期2255-2258,共4页Journal of Southern Medical University

基　　金：广东省医学科研基金(B2008031)

摘　　要：目的研究Notch1在食管鳞癌中的表达与临床病理特征的关系及其与食管鳞癌新生毛细血管的关系。方法通过免疫组化方法检测40例食管鳞癌和8例正常食管组织的Notch1和血管内皮生长因子(VEGF)、毛细血管密度(MVD)的表达情况并行相关性分析。结果Notch1在食管鳞癌组织中表达明显低于正常对照组(P<0.05),在不同的分化程度中有差异(P<0.05),但在肿瘤浸润深度和淋巴结转移与否中无明显差异(P>0.05)。VEGF和MVD在食管鳞癌组织中表达明显高于正常对照组(P<0.05),在不同分化程度、浸润深度和淋巴结转移与否中各组间均有显著性差异(P<0.05)。相关性分析显示Notch1与VEGF为负相关关系。结论Notch1在食管鳞癌中可能为抑癌基因;在早中期食管鳞癌中可能是主要影响肿瘤细胞的分化;其的异常低表达可能是引起VEGF和MVD异常高表达的原因之一。Notch信号通路在食管鳞癌新生毛细血管中可能有关键作用。Objective To observe Notchl expression in esophageal squamous cell carcinoma （ESCC） and investigate its relation with microvascular angiogenesis in the tumor. Methods Tissue slices of 40 cases ESCC （cancer group） and 8 cases normal esophagus tissues （normal group） were obtained to analyze the expression of Notchl and vascular endothelial growth factor （VEGF） using immunohistochemistry and estimate the microvessel density （MVD） in the tumor. Results Notchl expression was significantly lower in the cancer group than in the normal group （P〈0.05）. In the cancer group, Notchl expression was higher in highly differentiated than in poorly differentiated tumors （P〈0.05） regardless of tumor infiltration or lymph nodes metastasis （P〉0.05）. VEGF expression and MVD were significantly higher in cancer group than in normal group, and showed significant differences between tumors with different differentiation degrees, infiltration and lymph node metastasis （P〈0.05）. Correlation analysis showed that Notch/ expression was inversely correlated to VEGF expression. Conclusion Notchl may be an anti-oncogene in ESCC and affects cell differentiation in early stage of the malignancy. Abnormally low expression of Notchl in ESCC may be one of the upstream factors to induce high expression of VEGF and increased MVD. The Notchl pathway might play a key role in microvascular angiogenesis in ESCC.

关 键 词：NOTCH1 血管内皮生长因子 毛细血管密度 食管肿瘤 毛细血管 

分 类 号：R735.1[医药卫生—肿瘤]