富含多糖多酚芒果果肉组织总RNA的提取  

Isolation of total RNA from mango pulp tissue rich in polysaccharides and polyphenols

作　　者：张宇[1,2,3] 唐志鹏[1] 邓海燕[1] 王长江[1] 

机构地区：[1]广西大学农学院,广西南宁530004 [2]广西亚热带作物研究所,广西南宁530001 [3]广西农业科学院作物遗传改良与生物技术重点试验室,广西南宁530007

出　　处：《湖南农业大学学报（自然科学版）》2009年第6期637-639,共3页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：广西自然科学基金(桂科自08320338)

摘　　要：为寻求从芒果果肉组织提取总RNA的方法,以紫花芒果实为试材,在Tris-硼酸法基础上,在裂解液中加入聚乙烯吡咯烷酮除去干扰物质,在提取缓冲液中分别加入预冷的LiCl、异丙醇、无水乙醇沉淀剂,沉淀总RNA,结果表明,以异丙醇为沉淀剂的效果最理想,提取总RNA的获得率高,质量好,条带完整.该方法所提取的总RNA可用于RT-PCR、Northern杂交、cDNA文库构建等后续分子生物学研究.The fruit of Zihua mango （Mangifera indica L.） was used as experimental material to improve the method for isolation of total RNA from mango pulp. This method, based on the modified Tirs-HaBO4 method, was improved by adding polyvinylpyrrolidone into the extraction solution, and the pre-cooling LiCl, isopropanol and ethanol absolute into the precipitation buffer. The results showed that total RNA precipitated by isopropanol was better than the other precipitants. Total RNA isolated by this method was of high quality with high ratio of yield and a full strip, and was able to be used for later molecular manipulations such as RT-PCR, Northen hybrid and cDNA library construction.

关 键 词：芒果 果肉组织 总RNA 多糖 多酚 

分 类 号：S667.7[农业科学—果树学]