ADA-LacZ融合基因直接注射导入大鼠骨骼肌后的表达  

Expression of recombiant gene ADA LzcZ transfered by direct injection in the skeleton muscle of rat.

作　　者：杨仕林[1] 尤颖健[1] 屈伸[1] 张春明[1] 邓耀祖[1] 何善述[1] 肖同浩[1] 

机构地区：[1]同济医科大学基础医学院生化教研室 [2]广州军区武汉总医院心内科 [3]同济医科大学医学实验中心超微病理室

出　　处：《中国优生与遗传杂志》1998年第6期18-19,共2页Chinese Journal of Birth Health & Heredity

基　　金：卫生部和湖北省卫生厅科研基金

摘　　要：ＡＤＡ－ＬａｃＺ融合基因经直接注射导入大鼠骨骼肌内，用Ｘ－ｇａｌ和ＡＤＡ组化染色方法检测的结果表明：在大鼠骨骼肌细胞中，可检测到由ｐＦＰ表达的ＡＤＡ和β－ｇａｌ活性。这提示有望探索出一条对某些遗传性疾病、肿瘤及其它多种疾病导入外源基因实施基因治疗的便捷。ADA LacZ fusion gene was directly injected into the skeleton muscle of Wistar rat. The expression products were detected by immunohistochemical staining. The results showed that a biofunctional protein which possesses ADA and β galactosidase activites was encoded. It suggested that long sequence gene could be expressed in vivo by direct injection.

关 键 词：直接注射 融合基因 基因表达 骨骼肌 基因治疗 

分 类 号：R394[医药卫生—医学遗传学]