双极膜电渗析分离发酵液中L-乳酸  

Separation of L-lactic acid from fermentation broth by bipolar membrane electrodialysis

作　　者：李娟[1] 陈振[1] 王鹏[1] 姚黎明[1] 张腊梅[1] 王丽[1] 郑之明[1] 

机构地区：[1]中国科学院等离子体物理研究所离子束生物工程重点实验室,合肥230031

出　　处：《生物加工过程》2009年第6期45-50,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA020102);安徽省重点攻关资助项目(07010202076)

摘　　要：采用三室型双极膜电渗析装置将发酵液中的L-乳酸钠转化为L-乳酸。探讨操作电压、流速、进料L-乳酸钠质量浓度等工艺参数对转化过程的影响,考察电渗析过程参数对转化率、物料损失率、电流效率和能耗等技术指标的影响。在最优操作条件下(流速40 L/h,电压15 V)对2 L的100.25 g/L乳酸钠发酵液进行分批重复电渗析处理。结果表明:整个过程的转化率为81.22%,损失率为1.5%,能耗为0.81 kW.h/kg,电流效率为91.8%,得到的L-乳酸质量浓度可达144.31 g/L。电渗析残液补糖后可回到发酵罐中用于发酵生产L-乳酸。The separation of L-lactic acid was proposed from fermentation broth by bipolar membrane electrodialysis with three-chambers. Effects of operation voltage, flow rate and initial concentration of sodium L-lactate on some parameters were studied, such as conversion ratio of sodium lactate, loss rate, energy, consumption and current efficiency in the processes. Results showed that the final L-lactic acid concentration was 144. 31 g/L when fed-batch eleetrodialysis was performed to produce L-lactic acid from the broth containing 100. 25 g/L of sodium L-lactate under the optimum conditions of operation voltage at 15 V and flow rate at 40 L/h. The conversion ratio of sodium lactate, loss rate, energy consumption, and current efficiency were 81.22%, 1.5%, 0.81 kW·h/kg, and 91.8%. Electrodialysis waste water supplemented with glucose was reused as a fermentation medium for L-lactic acid production.

关 键 词：双极膜 电渗析 L-乳酸钠 L-乳酸 

分 类 号：TQ921.3[轻工技术与工程—发酵工程] Q851[生物学]