耐温性L-谷氨酸发酵菌种的选育  

Breeding of a thermotolerant Brevibacterium tianjinese for producing L-glutamic acid

作　　者：刘苗[1] 郑璞[1] 杜巧燕[1] 刘小德[1] 倪晔[1] 毛忠贵[1] 孙志浩[1] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214122

出　　处：《生物加工过程》2009年第6期74-78,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA020301-09)

摘　　要：应用基因组改组技术提高L-谷氨酸生产菌在高温发酵条件下的谷氨酸产量。以天津短杆菌T6-13变异株SW 07-1为原始亲株,分别经紫外线(UV)-硫酸二乙酯(DES)和X射线诱变,获得5株耐温性能略有提高的突变菌株。经2轮基因组改组,获得耐高温(能在44℃生长)的L-谷氨酸菌株F2-50。F2-50在38℃下,摇瓶发酵40 h,发酵液中L-谷氨酸浓度比原始出发菌株提高了近41%,在41℃高温下,摇瓶发酵40 h,L-谷氨酸浓度比原始出发菌株提高了近2倍。The breeding of thermotolerant L-glutamic acid producing strain by genome shuffling was investigated. A variant of Brevibacterium tianjinese T6-13, SW07-1 was used as the parent strain. Five mutants with subtle improvement in characteristic of heat resistance were generated by UV-DES （diethyl sulfate mutagenesis） and X-rays, respectively. A strain F2-50 capable of growing at 44 ~C and with higher L-glutamic acid productivity was obtained from the second round genome shuffled population. The L-glutamic acid production of F2-50 was 0.41 and 2 times higher than that of the initial strain at 38 ℃ and 40 ℃, respectively.

关 键 词：基因组改组 L-谷氨酸 耐温性 

分 类 号：Q933[生物学—微生物学]