GSTP1基因多态性与非小细胞肺癌遗传易感性和化疗敏感性的相关性研究  

GSTP1 gene polymorphism and susceptibility as well as chemotherapy sensitivity to non-small cell lung cancer

作　　者：岳峥[1] 徐倩[1] 徐莹[1] 董楠楠[1] 张晔[1] 朱丽波[2] 洪成雨[2] 袁媛[1] 

机构地区：[1]中国医科大学附属第一医院普通外科研究所暨肿瘤研究所第三研究室辽宁省高校肿瘤病因与预防重点实验室,辽宁沈阳110001 [2]辽宁省肿瘤医院内科,辽宁沈阳110042

出　　处：《中华肿瘤防治杂志》2009年第19期1441-1444,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30572131)

摘　　要：目的:探讨Ⅱ相代谢酶谷胱甘肽转硫酶GSTP1基因(105位点)多态性与非小细胞肺癌(non-small cell lung cancer,NSCLC)遗传易感性以及化疗敏感性关系。方法:采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对NSCLC患者和正常人群共204例检测外周血DNA GSTP1105位点的多态性;102例NSCLC患者选择NP方案化疗,化疗2个周期后评价疗效。结果:以A/A基因型为参照与其他2种G/G和A/G基因型比较,NSCLC GSTP1105位点分布频率差异无统计学意义,P>0.05。NSCLC患者按照性别、年龄、病理类型及临床分期1∶1配比后,以A/A基因型为参照与其他2种基因型比较,敏感组G/G+A/G分布频率(46.43%)高于非敏感组(21.43%),差异有统计学意义(χ2=3.95,P<0.05),携带A/G或G/G等位基因患者较携带A/A基因型者对化疗更为敏感。结论:GSTP1105位点多态性和NSCLC发病风险无明显关联。携带GSTP1105位点A/G或G/G基因型NSCLC患者,应用NP方案化疗敏感性高于携带A/A基因型患者。OBJECTIVE：To study the relationship between gene （105） polymorphism of phase Ⅱ metabolizing enzymes glutathione S-transferase GSTP1 and susceptibility to non-small cell lung cancer,and sensitivity to chemotherapy. METHODS：There were 204 cases of non-small cell lung cancer patients and the normal control persons,and by using a polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP）,the GSTP1 polymorphism was detected at 105 sites of DNA of peripheral blood of these cases. The 102 cases of non-small cell lung cancer patients chose NP chemotherapy,and the efficiency was evaluated two cycles after chemotherapy. RESULTS：Compared with A/A genotype,in the other two groups G/G and A/G genotype,GSTP1 105 distribution frequencies were of no statistically significant difference （P〉0.05）. Compared with A/A genotype,in the sensitive group,G/G＋A/G distribution frequency （46.47%） was higher than that in the non-sensitive group （21.43%）,and there was a statistically significant difference （χ2=3.95,P〈0.05）. Individuals with A/G or G/G allele,compared with patients with A/A genotype,were more sensitive to chemotherapy. CONCLUSIONS：There are no relevance between GSTP1 105 polymorphism and the risk of non-small cell lung cancer. The sensitivity of NP chemotherapy in cases of non-small cell lung cancer with GSTP1 AG or GG genotype is higher than that of patients with AA genotype.

关 键 词：谷胱甘肽转硫酶 多态现象 遗传 疾病遗传易感性 癌 非小细胞肺 

分 类 号：R734.2[医药卫生—肿瘤]