EGFR靶向纳米载体荷载c-erbB2反义寡脱氧核苷酸对人乳腺癌SK-BR3细胞的体外研究  

EGFR targeting nanoparticles loading c-erbB2 anti-oligodeoxynucleotides for SK-BR3 human breast cancer cells in vitro

作　　者：袁飞[1,2] 王树斌[3] 彭志平[4] 易发平[1] 卜友泉[1] 宋方洲(指导)[1] 李少林[4] 

机构地区：[1]重庆医科大学生物化学与分子生物学教研室,分子医学与肿瘤研究中心 [2]包头市中心医院检验科,内蒙古包头014040 [3]包头市中心医院肿瘤内科,内蒙古包头014040 [4]重庆医科大学放射医学教研室分子医学与肿瘤研究中心,重庆400016

出　　处：《第四军医大学学报》2009年第22期2515-2518,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30070230)

摘　　要：目的:探讨利用表皮生长因子偶联牛血清白蛋白纳米载体介导c-erbB2反义寡脱氧核苷酸以增加人乳腺癌SK-BR3细胞对其的摄取.方法:采用超声乳化-化学交联及羧和反应制备EGF偶联白蛋白靶向纳米载体.99mTc通过乙酰双半胱氨酸标记于c-erbB2反义寡脱氧核苷酸,研究其稳定性,结合靶基因的特异性,并探讨人乳腺癌SK-BR3细胞对其的摄取及滞留.结果:EGF靶向纳米载体包载99mTc-乙酰双半胱氨酸-c-erbB2反义寡脱氧核苷酸组的摄取率及滞留率均高于正义寡脱氧核苷酸组和无义寡脱氧核苷酸组.同时99mTc-乙酰双半胱氨酸-c-erbB2反义寡脱氧核苷酸使用纳米载体荷载组的摄取率及滞留率均高于未使用纳米载体组,差异有统计学意义(P<0.05).结论:EGF靶向纳米载体能够提高乳腺癌SK-BR3细胞对99mTc-乙酰双半胱氨酸-c-erbB2反义寡脱氧核苷酸的摄取和滞留,能达到更好的靶向作用.AIM：To investigate the better intake rate of human breast cancer cells SK-BR3 to c-erbB2 anti-oligodeoxynucleotides which was mediated by epidermal growth factor（EGF）coupling of bovine serum albumin（BSA）nanoparticles.METHODS：We usedultrasound emulsification-chemical cross-linking and carboxymethylreaction to prepare EGF-conjugated albumin targetingnanoparticles.^99mTc marked on the c-erbB2 anti-oligodeoxynucleotidesby paired with Acetyl double-Cysteine.The drug loading rate,encapsulation efficiency and the release rate were detected and calculated of EGF targeting nano-carrier loading c-erbB2 ASODN,and which uptake and stranded rates were exploredin SK-BR3 human breast cancer cells.RESULTS：The intake and retention rates in the EGF targeting nano-carrier packeting ^99mTc-antisense oligodeoxyribonucleotide（ASODN）group were higher than those in the 125I-sense oligodeoxynucleotide（SODN）group and the 99mTc-nonsense oligodeoxynucleotide（NSODN）group.At the same time,the uptake of retention rates in the c-erbB2 oligodeoxynucleotide with nano-carrier group were higher than those in the non-nano-carrier group（P〈0.05）.CONCLUSION：^99mTc-EGF-BSA targeting nano-carrier can increase the uptake and retention rates of c-erbB2 anti-oligodeoxynucleotides in breast cancer SK-BR3 cells,and achieve better targeting effects.

关 键 词：EGFR 牛血清白蛋白 C-ERBB2 纳米载体 

分 类 号：R73-3[医药卫生—肿瘤]