轻度认知功能障碍与ApoE基因多态性及血脂水平的研究  

Role of apolipoprotein E gene polymorphism and plasma lipids in mild cognitive impairment

作　　者：徐明明[1] 易咏红[1] 张萌[2] 

机构地区：[1]广州医学院第二附属医院神经科学研究所,广州510260 [2]广州医学院第一附属医院心内科,广州510120

出　　处：《山东大学学报（医学版）》2009年第11期103-107,共5页Journal of Shandong University:Health Sciences

摘　　要：目的分析轻度认知功能障碍(MCI)患者载脂蛋白E(ApoE)基因型及等位基因频率的分布及血脂水平,为MCI的防治提供理论依据。方法对120例MCI患者(MCI组)和120例健康者(对照组),进行简易精神状态检查表(MMSE)、日常生活行为量表(ADL)、总体衰退量表(GDS)、Hachinski缺血积分量表、汉密顿抑郁量表(HAMD)评测,采用聚合酶链反应-限制性片断长度多态性分析方法(PCR-RFLP),测定ApoE各基因型和等位基因频率并测定各例总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)水平。结果MCI组在时空定向、即刻记忆、延时记忆、注意计算力、理解力均较对照组差;ε3/4基因型频率及ε4等位基因频率均高于对照组(P<0.05);TC和LDL-C明显高于对照组(P<0.05),MCI组和对照组ε4组血脂均较ε3、ε2高(P<0.05)。结论ApoE基因型频率和等位基因频率在MCI组和对照组间的分布不同,ApoE基因可能是MCI的风险基因,MCI患者存在血脂紊乱,ApoEε4与MCI患者血脂紊乱有关。Objective To compare the distribution of Apolipoprotein E（ApoE） genotypes and plasma lipid levels between patients with mild cognitive impairment（MCI） and normal healthy people.Methods 120 patients with MCI and 120 normal controls were examined with a neuropsychological test which included minimental state examination（MMSE）,activities of daily living scale（ADL）,global deterioration scale（GDS） and Hachinski ischaemic scale（HIS）.Blood TC,TG,HDL-C and LDL-C were determined,and the ApoE phenotypes and allele frequency were determined using PCR-RFLP.Results MCI cases achieved significantly lower scores than normal controls in memory,execution function,attention,calculation,and directive force.Between the MCI and the control groups there was a statistical difference in ApoE genotypes and allele frequency.The ApoEε3/4 genotype and ApoEε4 in the MCI group were higher than those in the normal control（P〈0.05）.TC and LDL-C in the MCI group was higher than those in the normal controls（P〈0.05）.In the MCI and control groups,TC and LDL-C levels of ApoE ε4 were higher than those of ApoE ε3 and ApoE ε2（P〈0.05）.Conclusion ApoE genotypes and allele frequency are statistically different between the MCI and the control groups.ApoEε4 allele frequency is a risk factor of MCI.Dyslipidemia is found in MCI.

关 键 词：认知障碍 基因 载脂蛋白E 高脂血症 多态性 单核苷酸 

分 类 号：R749.16[医药卫生—神经病学与精神病学]